@article{88c1157140e848a692f303add95ee90c,
title = "Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds",
abstract = "X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.",
keywords = "3D tissue imaging, Arabidopsis imbibed seed, SPring-8, X-ray micro-CT, cell structure in seeds, ionic liquid",
author = "Daisuke Yamauchi and Aki Fukuda and Tomonori Nakai and Ichirou Karahara and Miyuki Takeuchi and Daisuke Tamaoki and Tetsuya Tsuda and Katsuhiko Tsunashima and Susumu Kuwabata and Masato Hoshino and Kentaro Uesugi and Akihisa Takeuchi and Yoshio Suzuki and Yoshinobu Mineyuki",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved.",
year = "2019",
month = feb,
day = "1",
doi = "10.1093/jmicro/dfy130",
language = "英語",
volume = "68",
pages = "92--97",
journal = "Microscopy",
issn = "2050-5698",
publisher = "Oxford University Press",
number = "1",
}