Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds

Daisuke Yamauchi; Aki Fukuda; Tomonori Nakai; Ichirou Karahara; Miyuki Takeuchi; Daisuke Tamaoki; Tetsuya Tsuda; Katsuhiko Tsunashima; Susumu Kuwabata; Masato Hoshino; Kentaro Uesugi; Akihisa Takeuchi; Yoshio Suzuki; Yoshinobu Mineyuki

doi:10.1093/jmicro/dfy130

Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds

Daisuke Yamauchi, Aki Fukuda, Tomonori Nakai, Ichirou Karahara, Miyuki Takeuchi, Daisuke Tamaoki, Tetsuya Tsuda, Katsuhiko Tsunashima, Susumu Kuwabata, Masato Hoshino, Kentaro Uesugi, Akihisa Takeuchi, Yoshio Suzuki, Yoshinobu Mineyuki^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.

Original language	English
Pages (from-to)	92-97
Number of pages	6
Journal	Microscopy
Volume	68
Issue number	1
DOIs	https://doi.org/10.1093/jmicro/dfy130
State	Published - 2019/02/01

Keywords

3D tissue imaging
Arabidopsis imbibed seed
SPring-8
X-ray micro-CT
cell structure in seeds
ionic liquid

ASJC Scopus subject areas

General Medicine

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10.1093/jmicro/dfy130

Cite this

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title = "Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds",

abstract = "X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.",

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AU - Yamauchi, Daisuke

AU - Fukuda, Aki

AU - Nakai, Tomonori

AU - Karahara, Ichirou

AU - Takeuchi, Miyuki

AU - Tamaoki, Daisuke

AU - Tsuda, Tetsuya

AU - Tsunashima, Katsuhiko

AU - Kuwabata, Susumu

AU - Hoshino, Masato

AU - Uesugi, Kentaro

AU - Takeuchi, Akihisa

AU - Suzuki, Yoshio

AU - Mineyuki, Yoshinobu

PY - 2019/2/1

Y1 - 2019/2/1

N2 - X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.

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KW - 3D tissue imaging

KW - Arabidopsis imbibed seed

KW - SPring-8

KW - X-ray micro-CT

KW - cell structure in seeds

KW - ionic liquid

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Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds

Abstract

Keywords

ASJC Scopus subject areas

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