Use of ionic liquid for X-ray micro-CT specimen preparation of imbibed seeds

Daisuke Yamauchi, Aki Fukuda, Tomonori Nakai, Ichirou Karahara, Miyuki Takeuchi, Daisuke Tamaoki, Tetsuya Tsuda, Katsuhiko Tsunashima, Susumu Kuwabata, Masato Hoshino, Kentaro Uesugi, Akihisa Takeuchi, Yoshio Suzuki, Yoshinobu Mineyuki*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

X-ray micro-CT is one of the most useful techniques to examine 3D cellular architecture inside dry seeds. However, the examination of imbibed seeds is difficult because immersion in water causes a decline in the image quality. Here, we examined the use of ionic liquids for specimen preparation of chemically fixed imbibed seeds of Arabidopsis. We found that treatment with high concentrations of ionic liquids after osmium tetroxide fixation helped not only to prevent the structural damage caused by seed shrinkage, but also to preserve the image quality. Under these conditions, the cellular architecture of seeds was also well maintained.

Original languageEnglish
Pages (from-to)92-97
Number of pages6
JournalMicroscopy
Volume68
Issue number1
DOIs
StatePublished - 2019/02/01

Keywords

  • 3D tissue imaging
  • Arabidopsis imbibed seed
  • SPring-8
  • X-ray micro-CT
  • cell structure in seeds
  • ionic liquid

ASJC Scopus subject areas

  • General Medicine

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