Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Mst Ayesha Siddika; Takahiro Yamada; Risako Aoyama; Kumi Hidaka; Hiroshi Sugiyama; Masayuki Endo; Shigeyoshi Matsumura; Yoshiya Ikawa

doi:10.3390/molecules27238298

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Mst Ayesha Siddika, Takahiro Yamada, Risako Aoyama, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa^*

^*この論文の責任著者

理学科　化学プログラム

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

1 被引用数 (Scopus)

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Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

本文言語	英語
論文番号	8298
ジャーナル	Molecules
巻	27
号	23
DOI	https://doi.org/10.3390/molecules27238298
出版ステータス	出版済み - 2022/12

ASJC Scopus 主題領域

分析化学
化学（その他）
分子医療
薬科学
創薬
物理化学および理論化学
有機化学

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10.3390/molecules27238298

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title = "Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes",

abstract = "Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).",

keywords = "RNA motif, RNA nanostructure, pre-tRNA processing, ribonuclease P, ribozyme",

author = "Siddika, {Mst Ayesha} and Takahiro Yamada and Risako Aoyama and Kumi Hidaka and Hiroshi Sugiyama and Masayuki Endo and Shigeyoshi Matsumura and Yoshiya Ikawa",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

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month = dec,

doi = "10.3390/molecules27238298",

language = "英語",

volume = "27",

journal = "Molecules",

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T1 - Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

AU - Siddika, Mst Ayesha

AU - Yamada, Takahiro

AU - Aoyama, Risako

AU - Hidaka, Kumi

AU - Sugiyama, Hiroshi

AU - Endo, Masayuki

AU - Matsumura, Shigeyoshi

AU - Ikawa, Yoshiya

PY - 2022/12

Y1 - 2022/12

N2 - Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

AB - Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

KW - RNA motif

KW - RNA nanostructure

KW - pre-tRNA processing

KW - ribonuclease P

KW - ribozyme

UR - http://www.scopus.com/inward/record.url?scp=85143710242&partnerID=8YFLogxK

U2 - 10.3390/molecules27238298

DO - 10.3390/molecules27238298

M3 - 学術論文

C2 - 36500390

AN - SCOPUS:85143710242

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 23

M1 - 8298

ER -

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

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