Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Mst Ayesha Siddika; Takahiro Yamada; Risako Aoyama; Kumi Hidaka; Hiroshi Sugiyama; Masayuki Endo; Shigeyoshi Matsumura; Yoshiya Ikawa

doi:10.3390/molecules27238298

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Mst Ayesha Siddika, Takahiro Yamada, Risako Aoyama, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa^*

^*Corresponding author for this work

Program of Chemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

Original language	English
Article number	8298
Journal	Molecules
Volume	27
Issue number	23
DOIs	https://doi.org/10.3390/molecules27238298
State	Published - 2022/12

Keywords

RNA motif
RNA nanostructure
pre-tRNA processing
ribonuclease P
ribozyme

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

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10.3390/molecules27238298

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title = "Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes",

abstract = "Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).",

keywords = "RNA motif, RNA nanostructure, pre-tRNA processing, ribonuclease P, ribozyme",

author = "Siddika, {Mst Ayesha} and Takahiro Yamada and Risako Aoyama and Kumi Hidaka and Hiroshi Sugiyama and Masayuki Endo and Shigeyoshi Matsumura and Yoshiya Ikawa",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

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doi = "10.3390/molecules27238298",

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volume = "27",

journal = "Molecules",

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T1 - Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

AU - Siddika, Mst Ayesha

AU - Yamada, Takahiro

AU - Aoyama, Risako

AU - Hidaka, Kumi

AU - Sugiyama, Hiroshi

AU - Endo, Masayuki

AU - Matsumura, Shigeyoshi

AU - Ikawa, Yoshiya

PY - 2022/12

Y1 - 2022/12

N2 - Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

AB - Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization. The resulting RNA nanostructures can exhibit the catalytic function of the parent ribozyme in an assembly dependent manner. In this study, we designed and constructed open-form oligomers of a bimolecular form of an RNase P ribozyme. The ribozyme oligomers were analyzed biochemically and by atomic force microscopy (AFM).

KW - RNA motif

KW - RNA nanostructure

KW - pre-tRNA processing

KW - ribonuclease P

KW - ribozyme

UR - http://www.scopus.com/inward/record.url?scp=85143710242&partnerID=8YFLogxK

U2 - 10.3390/molecules27238298

DO - 10.3390/molecules27238298

M3 - 学術論文

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AN - SCOPUS:85143710242

SN - 1420-3049

VL - 27

JO - Molecules

JF - Molecules

IS - 23

M1 - 8298

ER -

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Abstract

Keywords

ASJC Scopus subject areas

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