Abstract
An acidic environment in bone is essential for bone metabolism and the production of decarboxylated osteocalcin, which functions as a regulatory hormone of glucose metabolism. Here, we describe the high-resolution X-ray crystal structure of decarboxylated osteocalcin under acidic conditions. Decarboxylated osteocalcin at pH 2.0 retains the α-helix structure of native osteocalcin with three γ-carboxyglutamic acid residues at neutral pH. This implies that decarboxylated osteocalcin is stable under an acidic environment in bone. In addition, site-directed mutagenesis revealed that Glu17 and Glu21 are important for the adiponectin-inducing activity of decarboxylated osteocalcin. These findings suggest that the receptor of decarboxylated osteocalcin responds to the negative charge in helix 1 of osteocalcin.
Original language | English |
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Pages (from-to) | 1479-1488 |
Number of pages | 10 |
Journal | FEBS Letters |
Volume | 597 |
Issue number | 11 |
DOIs | |
State | Published - 2023/06 |
Keywords
- GPRC6A
- adiponectin
- adiponectin-inducing activity
- decarboxylated osteocalcin
- glucose metabolism
- helix
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology