Protonation State and Hydration of Bisphosphonate Bound to Farnesyl Pyrophosphate Synthase

Takeshi Yokoyama*, Mineyuki Mizuguchi, Andreas Ostermann, Katsuhiro Kusaka, Nobuo Niimura, Tabias E. Schrader, Ichiro Tanaka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Farnesyl pyrophosphate synthase (FPPS) catalyzes the condensation of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate to FPP and is known to be a molecular target of osteoporosis drugs, such as risedronate (RIS), which is a nitrogen-containing bisphosphonate. The protonation states and hydration structure of RIS bound to FPPS were determined by neutron protein crystallography, which allows direct visualization of hydrogens and deuteriums. The structure analysis revealed that the phosphate groups of RIS were fully deprotonated with the abnormally decreased pKa, and that the roles of E93 and D264 consisted of canceling the extra negative charges upon the binding of ligands. Collectively, our neutron structures provided insights into the physicochemical properties during the bisphosphonate binding event.

Original languageEnglish
Pages (from-to)7549-7556
Number of pages8
JournalJournal of Medicinal Chemistry
Volume58
Issue number18
DOIs
StatePublished - 2015/09/24

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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