Characterization of the interaction between Trypanosoma brucei Pex5p and its receptor Pex14p

Yuichi Watanabe; Kosuke Kawaguchi; Naoki Okuyama; Yuri Sugawara; Takayuki Obita; Mineyuki Mizuguchi; Masashi Morita; Tsuneo Imanaka

doi:10.1002/1873-3468.12044

Characterization of the interaction between Trypanosoma brucei Pex5p and its receptor Pex14p

Yuichi Watanabe, Kosuke Kawaguchi, Naoki Okuyama, Yuri Sugawara, Takayuki Obita, Mineyuki Mizuguchi, Masashi Morita, Tsuneo Imanaka^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The interaction of Trypanosoma brucei (Tb) Pex5p and its receptor TbPex14p is essential for the translocation of newly synthesized matrix proteins into the glycosome. Here, we reveal that only the third WXXXF/Y motif of TbPex5p is involved in the interaction and that negative charge of the fourth amino acid is important. We suggest that Phe35 and Phe52 of TbPex14p interact with Trp318 and Phe322 in the third motif and that the Lys56 adjacent to Phe35/Phe52 associates with the fourth Glu in the motif to make the complex. This information is expected to be useful for developing anti-trypanosomal drugs.

Original language	English
Pages (from-to)	242-250
Number of pages	9
Journal	FEBS Letters
Volume	590
Issue number	2
DOIs	https://doi.org/10.1002/1873-3468.12044
State	Published - 2016/01/01

Keywords

Pex14p
Pex5p
WXXXF/Y motif
biogenesis
glycosome
trypanosome

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1002/1873-3468.12044

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title = "Characterization of the interaction between Trypanosoma brucei Pex5p and its receptor Pex14p",

abstract = "The interaction of Trypanosoma brucei (Tb) Pex5p and its receptor TbPex14p is essential for the translocation of newly synthesized matrix proteins into the glycosome. Here, we reveal that only the third WXXXF/Y motif of TbPex5p is involved in the interaction and that negative charge of the fourth amino acid is important. We suggest that Phe35 and Phe52 of TbPex14p interact with Trp318 and Phe322 in the third motif and that the Lys56 adjacent to Phe35/Phe52 associates with the fourth Glu in the motif to make the complex. This information is expected to be useful for developing anti-trypanosomal drugs.",

keywords = "Pex14p, Pex5p, WXXXF/Y motif, biogenesis, glycosome, trypanosome",

author = "Yuichi Watanabe and Kosuke Kawaguchi and Naoki Okuyama and Yuri Sugawara and Takayuki Obita and Mineyuki Mizuguchi and Masashi Morita and Tsuneo Imanaka",

note = "Publisher Copyright: {\textcopyright} 2015 Federation of European Biochemical Societies.",

year = "2016",

month = jan,

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doi = "10.1002/1873-3468.12044",

language = "英語",

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T1 - Characterization of the interaction between Trypanosoma brucei Pex5p and its receptor Pex14p

AU - Watanabe, Yuichi

AU - Kawaguchi, Kosuke

AU - Okuyama, Naoki

AU - Sugawara, Yuri

AU - Obita, Takayuki

AU - Mizuguchi, Mineyuki

AU - Morita, Masashi

AU - Imanaka, Tsuneo

PY - 2016/1/1

Y1 - 2016/1/1

N2 - The interaction of Trypanosoma brucei (Tb) Pex5p and its receptor TbPex14p is essential for the translocation of newly synthesized matrix proteins into the glycosome. Here, we reveal that only the third WXXXF/Y motif of TbPex5p is involved in the interaction and that negative charge of the fourth amino acid is important. We suggest that Phe35 and Phe52 of TbPex14p interact with Trp318 and Phe322 in the third motif and that the Lys56 adjacent to Phe35/Phe52 associates with the fourth Glu in the motif to make the complex. This information is expected to be useful for developing anti-trypanosomal drugs.

AB - The interaction of Trypanosoma brucei (Tb) Pex5p and its receptor TbPex14p is essential for the translocation of newly synthesized matrix proteins into the glycosome. Here, we reveal that only the third WXXXF/Y motif of TbPex5p is involved in the interaction and that negative charge of the fourth amino acid is important. We suggest that Phe35 and Phe52 of TbPex14p interact with Trp318 and Phe322 in the third motif and that the Lys56 adjacent to Phe35/Phe52 associates with the fourth Glu in the motif to make the complex. This information is expected to be useful for developing anti-trypanosomal drugs.

KW - Pex14p

KW - Pex5p

KW - WXXXF/Y motif

KW - biogenesis

KW - glycosome

KW - trypanosome

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U2 - 10.1002/1873-3468.12044

DO - 10.1002/1873-3468.12044

M3 - 学術論文

C2 - 26762183

AN - SCOPUS:84957310331

SN - 0014-5793

VL - 590

SP - 242

EP - 250

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Characterization of the interaction between Trypanosoma brucei Pex5p and its receptor Pex14p

Abstract

Keywords

ASJC Scopus subject areas

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