Segmental labeling of the intrinsically disordered protein PQBP1 and elucidation of the molecular recognition mechanism by NMR

Project Details

Description

We successfully prepared segmentally labeled PQBP1, in which the C-terminal domain is 15N-labeled. Two fragments of PQBP1 were highly expressed in E. coli with the cold-shock vector, and ligated by the expressed protein ligation method. Our results will be applicable to the segmental labeling of other intrinsically disordered proteins.
StatusFinished
Effective start/end date2009/04/012012/03/31

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