Project Details
Description
We successfully prepared segmentally labeled PQBP1, in which the C-terminal domain is 15N-labeled. Two fragments of PQBP1 were highly expressed in E. coli with the cold-shock vector, and ligated by the expressed protein ligation method. Our results will be applicable to the segmental labeling of other intrinsically disordered proteins.
Status | Finished |
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Effective start/end date | 2009/04/01 → 2012/03/31 |
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