Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action

Masahiko Inouye*, Reona Ikeda, Masayoshi Takase, Takashi Tsuri, Junya Chiba

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

101 被引用数 (SciVal)

抄録

Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, "wire-like" DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved "on-off"-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, "hot spots" from the p53 gene, could clearly be distinguished from wild type by using our methodology.

本文言語英語
ページ(範囲)11606-11610
ページ数5
ジャーナルProceedings of the National Academy of Sciences of the United States of America
102
33
DOI
出版ステータス出版済み - 2005/08/16

ASJC Scopus 主題領域

  • 一般

フィンガープリント

「Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル