抄録
Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, "wire-like" DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved "on-off"-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, "hot spots" from the p53 gene, could clearly be distinguished from wild type by using our methodology.
本文言語 | 英語 |
---|---|
ページ(範囲) | 11606-11610 |
ページ数 | 5 |
ジャーナル | Proceedings of the National Academy of Sciences of the United States of America |
巻 | 102 |
号 | 33 |
DOI | |
出版ステータス | 出版済み - 2005/08/16 |
ASJC Scopus 主題領域
- 一般