Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action

Masahiko Inouye; Reona Ikeda; Masayoshi Takase; Takashi Tsuri; Junya Chiba

doi:10.1073/pnas.0502078102

Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action

Masahiko Inouye^*, Reona Ikeda, Masayoshi Takase, Takashi Tsuri, Junya Chiba

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

101 Citations (SciVal)

Abstract

Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, "wire-like" DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved "on-off"-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, "hot spots" from the p53 gene, could clearly be distinguished from wild type by using our methodology.

Original language	English
Pages (from-to)	11606-11610
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	33
DOIs	https://doi.org/10.1073/pnas.0502078102
State	Published - 2005/08/16

Keywords

Electrochemistry
Ferrocene
Gold electrode
Hole transport

ASJC Scopus subject areas

General

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10.1073/pnas.0502078102

Cite this

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title = "Single-nucleotide polymorphism detection with {"}wire-like{"} DNA probes that display quasi {"}on-off{"} digital action",

abstract = "Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, {"}wire-like{"} DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved {"}on-off{"}-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, {"}hot spots{"} from the p53 gene, could clearly be distinguished from wild type by using our methodology.",

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Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action. / Inouye, Masahiko; Ikeda, Reona; Takase, Masayoshi et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 33, 16.08.2005, p. 11606-11610.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action

AU - Inouye, Masahiko

AU - Ikeda, Reona

AU - Takase, Masayoshi

AU - Tsuri, Takashi

AU - Chiba, Junya

PY - 2005/8/16

Y1 - 2005/8/16

N2 - Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, "wire-like" DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved "on-off"-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, "hot spots" from the p53 gene, could clearly be distinguished from wild type by using our methodology.

AB - Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, "wire-like" DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved "on-off"-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, "hot spots" from the p53 gene, could clearly be distinguished from wild type by using our methodology.

KW - Electrochemistry

KW - Ferrocene

KW - Gold electrode

KW - Hole transport

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U2 - 10.1073/pnas.0502078102

DO - 10.1073/pnas.0502078102

M3 - 学術論文

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AN - SCOPUS:23844431945

SN - 0027-8424

VL - 102

SP - 11606

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 33

ER -

Single-nucleotide polymorphism detection with "wire-like" DNA probes that display quasi "on-off" digital action

Abstract

Keywords

ASJC Scopus subject areas

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