Seasonal variations in airborne bacterial community structures at a suburban site of central Japan over a 1-year time period using PCR-DGGE method

Daisuke Tanaka*, Yohei Terada, Takuya Nakashima, Akihiro Sakatoku, Shogo Nakamura

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

26 被引用数 (Scopus)

抄録

Airborne bacteria could affect human health and climate. In this study, the bacterial community structures were investigated by analyzing air samples collected from a suburban location in Toyama, central Japan. Sixty samples were collected almost weekly between March 2009 and February 2010. A cultivation-independent approach, PCR plus denaturing gradient gel electrophoresis (DGGE), was used to analyze changes in the structure of the airborne bacterial communities. The DGGE profiles showed significant differences between sampling days, reflecting quite different bacterial profiles. Sequencing of DGGE bands revealed that the bacterial community was dominated by γ-Proteobacteria, Firmicutes, α-Proteobacteria, Bacteroidetes, and Actinobacteria. The most frequently observed genus was Pseudomonas, followed by Bacillus. The airborne bacterial assemblage in winter differed from that in the other seasons. The differences could be attributed a number of factors, such as temperature, relative humidity, wind direction, and snowfall. In spring, the largest number of bands was classified as plant chloroplasts, such as Japanese cedar (Cryptomeria japonica) and Pinus spp., which are the major pollen allergen sources in Japan. The airborne bacterial abundance estimated by real-time TaqMan PCR ranged from 1.1 × 103 to 1.3 × 105 cells m−3.

本文言語英語
ページ(範囲)143-157
ページ数15
ジャーナルAerobiologia
31
2
DOI
出版ステータス出版済み - 2015/06/01

ASJC Scopus 主題領域

  • 免疫アレルギー学
  • 免疫学
  • 植物科学

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