TY - CHAP
T1 - Isolation of antigen-specific, antibody-secreting cells using a chip-based immunospot array
AU - Kishi, Hiroyuki
AU - Ozawa, Tatsuhiko
AU - Hamana, Hiroshi
AU - Kobayashi, Eiji
AU - Muraguchi, Atsushi
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used to produce antigen-specific monoclonal antibodies. However, a good hybridoma system is not available to obtain human monoclonal antibodies. To produce antigen-specific human monoclonal antibodies, transformation of B lymphocytes with Epstein-Barr viruses or a phage-display system is used. Here, we describe the screening of antigen-specific, antibody-secreting cells using microwell array chips to obtain antigen-specific human monoclonal antibodies. The system can be applied to screen antigen-specific, antibody-secreting cells from any animal species.
AB - Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used to produce antigen-specific monoclonal antibodies. However, a good hybridoma system is not available to obtain human monoclonal antibodies. To produce antigen-specific human monoclonal antibodies, transformation of B lymphocytes with Epstein-Barr viruses or a phage-display system is used. Here, we describe the screening of antigen-specific, antibody-secreting cells using microwell array chips to obtain antigen-specific human monoclonal antibodies. The system can be applied to screen antigen-specific, antibody-secreting cells from any animal species.
KW - Antibody-secreting cell
KW - Antigen-specific antibody
KW - Immunospot array assay on a chip
KW - Microwell-array chip
UR - http://www.scopus.com/inward/record.url?scp=85058595565&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-8958-4_6
DO - 10.1007/978-1-4939-8958-4_6
M3 - 章
C2 - 30539469
AN - SCOPUS:85058595565
T3 - Methods in Molecular Biology
SP - 147
EP - 162
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -