Isolation of antigen-specific, antibody-secreting cells using a chip-based immunospot array

Hiroyuki Kishi*, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, Atsushi Muraguchi

*この論文の責任著者

研究成果: 書籍の章/レポート/会議録査読

1 被引用数 (Scopus)

抄録

Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used to produce antigen-specific monoclonal antibodies. However, a good hybridoma system is not available to obtain human monoclonal antibodies. To produce antigen-specific human monoclonal antibodies, transformation of B lymphocytes with Epstein-Barr viruses or a phage-display system is used. Here, we describe the screening of antigen-specific, antibody-secreting cells using microwell array chips to obtain antigen-specific human monoclonal antibodies. The system can be applied to screen antigen-specific, antibody-secreting cells from any animal species.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ147-162
ページ数16
DOI
出版ステータス出版済み - 2019

出版物シリーズ

名前Methods in Molecular Biology
1904
ISSN(印刷版)1064-3745

ASJC Scopus 主題領域

  • 分子生物学
  • 遺伝学

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