Isolation of antigen-specific, antibody-secreting cells using a chip-based immunospot array

Hiroyuki Kishi; Tatsuhiko Ozawa; Hiroshi Hamana; Eiji Kobayashi; Atsushi Muraguchi

doi:10.1007/978-1-4939-8958-4_6

Isolation of antigen-specific, antibody-secreting cells using a chip-based immunospot array

Hiroyuki Kishi^*, Tatsuhiko Ozawa, Hiroshi Hamana, Eiji Kobayashi, Atsushi Muraguchi

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

Antigen-specific monoclonal antibodies are useful tools to detect very small amounts of antigenic materials and are applicable for antibody therapeutics. To produce mouse monoclonal antibodies, a hybridoma between B lymphocytes and myeloma cells is used to produce antigen-specific monoclonal antibodies. However, a good hybridoma system is not available to obtain human monoclonal antibodies. To produce antigen-specific human monoclonal antibodies, transformation of B lymphocytes with Epstein-Barr viruses or a phage-display system is used. Here, we describe the screening of antigen-specific, antibody-secreting cells using microwell array chips to obtain antigen-specific human monoclonal antibodies. The system can be applied to screen antigen-specific, antibody-secreting cells from any animal species.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	147-162
Number of pages	16
DOIs	https://doi.org/10.1007/978-1-4939-8958-4_6
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1904
ISSN (Print)	1064-3745

Keywords

Antibody-secreting cell
Antigen-specific antibody
Immunospot array assay on a chip
Microwell-array chip

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-8958-4_6

Cite this

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AU - Muraguchi, Atsushi

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Isolation of antigen-specific, antibody-secreting cells using a chip-based immunospot array

Abstract

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Keywords

ASJC Scopus subject areas

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