Heterodimerization of Group I Ribozymes Enabling Exon Recombination through Pairs of Cooperative trans-Splicing Reactions

Takahiro Tanaka, Yusuke Hirata, Yuto Tominaga, Hiroyuki Furuta, Shigeyoshi Matsumura, Yoshiya Ikawa*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

9 被引用数 (Scopus)

抄録

Group I (GI) self-splicing ribozymes are attractive tools for biotechnology and synthetic biology. Several trans-splicing and related reactions based on GI ribozymes have been developed for the purpose of recombining their target mRNA sequences. By combining trans-splicing systems with rational modular engineering of GI ribozymes it was possible to achieve more complex editing of target RNA sequences. In this study we have developed a cooperative trans-splicing system through rational modular engineering with use of dimeric GI ribozymes derived from the Tetrahymena group I intron ribozyme. The resulting pairs of ribozymes exhibited catalytic activity depending on their selective dimerization. Rational modular redesign as performed in this study would facilitate the development of sophisticated regulation of double or multiple trans-splicing reactions in a cooperative manner.

本文言語英語
ページ(範囲)1659-1667
ページ数9
ジャーナルChemBioChem
18
16
DOI
出版ステータス出版済み - 2017/08/17

ASJC Scopus 主題領域

  • 生化学
  • 分子医療
  • 分子生物学
  • 有機化学

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