Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

Paras Jawaid, Mati Ur Rehman, Yoko Yoshihisa, Peng Li, Qing Li Zhao, Mariame Ali Hassan, Yusei Miyamoto, Tadamichi Shimizu, Takashi Kondo*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

49 被引用数 (Scopus)

抄録

Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.

本文言語英語
ページ(範囲)1006-1016
ページ数11
ジャーナルApoptosis
19
6
DOI
出版ステータス出版済み - 2014/06

ASJC Scopus 主題領域

  • 薬理学
  • 薬科学
  • 臨床生化学
  • 細胞生物学
  • 生化学、医学
  • 癌研究

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