Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

Paras Jawaid; Mati Ur Rehman; Yoko Yoshihisa; Peng Li; Qing Li Zhao; Mariame Ali Hassan; Yusei Miyamoto; Tadamichi Shimizu; Takashi Kondo

doi:10.1007/s10495-014-0972-5

Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

Paras Jawaid, Mati Ur Rehman, Yoko Yoshihisa, Peng Li, Qing Li Zhao, Mariame Ali Hassan, Yusei Miyamoto, Tadamichi Shimizu, Takashi Kondo^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O₂^-) and hydrogen peroxide (H₂O₂), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H ₂O₂), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O ₂^- formation.

Original language	English
Pages (from-to)	1006-1016
Number of pages	11
Journal	Apoptosis
Volume	19
Issue number	6
DOIs	https://doi.org/10.1007/s10495-014-0972-5
State	Published - 2014/06

Keywords

Apoptosis
Platinum nano-particles
Radiation
Reactive Oxygen Species (ROS)

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science
Clinical Biochemistry
Cell Biology
Biochemistry, medical
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10495-014-0972-5

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title = "Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells",

abstract = "Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.",

keywords = "Apoptosis, Platinum nano-particles, Radiation, Reactive Oxygen Species (ROS)",

author = "Paras Jawaid and Rehman, {Mati Ur} and Yoko Yoshihisa and Peng Li and Zhao, {Qing Li} and Hassan, {Mariame Ali} and Yusei Miyamoto and Tadamichi Shimizu and Takashi Kondo",

year = "2014",

month = jun,

doi = "10.1007/s10495-014-0972-5",

language = "英語",

volume = "19",

pages = "1006--1016",

journal = "Apoptosis",

issn = "1360-8185",

publisher = "Springer",

number = "6",

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TY - JOUR

T1 - Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

AU - Jawaid, Paras

AU - Rehman, Mati Ur

AU - Yoshihisa, Yoko

AU - Li, Peng

AU - Zhao, Qing Li

AU - Hassan, Mariame Ali

AU - Miyamoto, Yusei

AU - Shimizu, Tadamichi

AU - Kondo, Takashi

PY - 2014/6

Y1 - 2014/6

N2 - Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.

AB - Since polyacrylic acid capped platinum nanoparticles (nano-Pts) are known to have a unique ability to quench superoxide (O2-) and hydrogen peroxide (H2O2), the anti-oxidant activity of nano-Pts against apoptosis induced by x-irradiation in human lymphoma U937 cells was investigated. DNA fragmentation assay, Annexin V-FITC/PI by flow cytometry and Giemsa staining revealed a significant decrease in apoptosis induced by 10 Gy, when cells were pre-treated with nano-Pts in a dose-dependent manner. Pre-treatment with nano-Pts significantly decreased radiation-induced reactive oxygen species (ROS) production, Fas expression and loss of mitochondrial membrane potential as determined by flow-cytometry. Furthermore, western blot analysis also showed that the expression of cleaved caspase-3, Bid and cytosolic cytochrome- c were significantly reduced in nano-Pts pretreated cells. Due to the catalase mimetic activity of nano-Pts, these results indicate that pre-treatment of U937 cells with nano-Pts significantly protect radiation-induced apoptosis by inhibiting intracellular ROS (mainly H 2O2), which plays a key role in the induction of apoptosis, because of no practical observation of intracellular O 2- formation.

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KW - Platinum nano-particles

KW - Radiation

KW - Reactive Oxygen Species (ROS)

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U2 - 10.1007/s10495-014-0972-5

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Effects of SOD/catalase mimetic platinum nanoparticles on radiation-induced apoptosis in human lymphoma U937 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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