DNA microarray analysis of differentially expressed genes responsive to bisphenol A, an alkylphenol derivative, in an in vitro mouse sertoli cell model

To identify genes elicited by bisphenol A (BPA) in Sertoli cells, we carried out a microarray analysis of TTE3 cells (a mouse Sertoli cell line) treated with BPA. BPA (100, 200 and 400 μM) induced cell death concentration-dependently, with levels being 25%, 33% and 96%, respectively. Of the 1,081 genes analyzed, 3 genes showed decreased levels of expression while the remaining 10 genes showed increased levels in the cells treated with a subtoxic dose of BPA (200 μM). The expressions of six genes were confirmed by the TaqMan assay. These findings suggest that DNA microarray analysis is a useful tool for investigating the molecular mechanisms of the toxic effects of BPA in testicular cells.