@article{a5e0784b06054698a273e8add95596db,
title = "Coordinated control of a designed trans-acting ligase ribozyme by a loop-receptor interaction",
abstract = "We previously developed a synthetic cis-acting RNA ligase ribozyme with 3′-5′ joining activity termed {"}DSL{"} (designed and selected ligase). DSL was easily transformed into a trans-acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans-acting DSL, tDSL-1/GUAA. tDSL-1/GUAA exhibited remarkably high activity compared with the parental cis-acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop-receptor interaction plays a significant role in determining the activity of the trans-acting ribozyme and in its ability to perform multiple turnovers of the reaction.",
keywords = "DSL ribozyme, Loop-receptor interaction, Modular engineering, Multiple-turnover, RNA ligase ribozyme, Trans-acting ribozyme",
author = "Shigeyoshi Matsumura and Rei Ohmori and Hirohide Saito and Yoshiya Ikawa and Tan Inoue",
note = "Funding Information: We thank the members of the Inoue Laboratory for helpful comments. This work was supported by Grants-in-Aid for Scientific Research on Priority Areas (T.I.) from the Ministry of Education, Science, Sports and Culture, Japan, ICORP from JST (T.I., H.S.), and Japan Society for the Promotion of Science Research Fellowships for Young Scientists (S.M.). ",
year = "2009",
month = sep,
day = "3",
doi = "10.1016/j.febslet.2009.07.036",
language = "英語",
volume = "583",
pages = "2819--2826",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "17",
}