Biochemical Characterization of a Non-G4-Type RNA Aptamer That Lights Up a GFP-like Fluorogenic Ligand

Shunsuke Abe, Shino Aburaya, Takaki Koyama, Takashi Usui, Junro Yoshino, Shigeyoshi Matsumura, Yoshiya Ikawa*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

抄録

The 17-3 RNA aptamer recognizes DMHBI and induces its fluorescence. We showed that the 17-3 RNA aptamer predominantly induced emission of the phenolate form of DMHBI. We also demonstrated that the active structure of the minimal form of the 17-3 aptamer possessed three stem elements and two large loop elements, which we named Karashi and its sequence-optimized variant, Jigarashi, respectively. Chemical modification experiments suggested that the two loop regions formed tertiary interactions and/or non-Watson–Crick base pairs, and no remarkable structural alterations occurred upon DMHBI binding. AlphaFold3 also predicted a tertiary structure of the ligand-free form of Jigarashi RNA, which was consistent with the results of chemical modification experiments.

本文言語英語
論文番号1777
ジャーナルMolecules
30
8
DOI
出版ステータス出版済み - 2025/04

ASJC Scopus 主題領域

  • 分析化学
  • 化学(その他)
  • 分子医療
  • 薬科学
  • 創薬
  • 物理化学および理論化学
  • 有機化学

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