抄録
The 17-3 RNA aptamer recognizes DMHBI and induces its fluorescence. We showed that the 17-3 RNA aptamer predominantly induced emission of the phenolate form of DMHBI. We also demonstrated that the active structure of the minimal form of the 17-3 aptamer possessed three stem elements and two large loop elements, which we named Karashi and its sequence-optimized variant, Jigarashi, respectively. Chemical modification experiments suggested that the two loop regions formed tertiary interactions and/or non-Watson–Crick base pairs, and no remarkable structural alterations occurred upon DMHBI binding. AlphaFold3 also predicted a tertiary structure of the ligand-free form of Jigarashi RNA, which was consistent with the results of chemical modification experiments.
本文言語 | 英語 |
---|---|
論文番号 | 1777 |
ジャーナル | Molecules |
巻 | 30 |
号 | 8 |
DOI | |
出版ステータス | 出版済み - 2025/04 |
ASJC Scopus 主題領域
- 分析化学
- 化学(その他)
- 分子医療
- 薬科学
- 創薬
- 物理化学および理論化学
- 有機化学