A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

Tatsuhiko Ozawa; Xiuhong Piao; Eiji Kobayashi; Yue Zhou; Hiroaki Sakurai; Tsugunobu Andoh; Aishun Jin; Hiroyuki Kishi; Atsushi Muraguchi

doi:10.1371/journal.pone.0052383

A Novel Rabbit Immunospot Array Assay on a Chip Allows for the Rapid Generation of Rabbit Monoclonal Antibodies with High Affinity

Tatsuhiko Ozawa, Xiuhong Piao, Eiji Kobayashi, Yue Zhou, Hiroaki Sakurai, Tsugunobu Andoh, Aishun Jin, Hiroyuki Kishi^*, Atsushi Muraguchi

^*この論文の責任著者

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

25 被引用数 (Scopus)

抄録

Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their high specificity and high affinity, and the establishment of a comprehensive and rapid RaMoAb generation system has been highly anticipated. Here, we present a novel system using immunospot array assay on a chip (ISAAC) technology in which we detect and retrieve antigen-specific antibody-secreting cells from the peripheral blood lymphocytes of antigen-immunized rabbits and produce antigen-specific RaMoAbs with 10^-12 M affinity within a time period of only 7 days. We have used this system to efficiently generate RaMoAbs that are specific to a phosphorylated signal-transducing molecule. Our system provides a new method for the comprehensive and rapid production of RaMoAbs, which may contribute to laboratory research and clinical applications.

本文言語	英語
論文番号	e52383
ジャーナル	PLoS ONE
巻	7
号	12
DOI	https://doi.org/10.1371/journal.pone.0052383
出版ステータス	出版済み - 2012/12/29

ASJC Scopus 主題領域

生化学、遺伝学、分子生物学一般
農業および生物科学一般
一般

文献へのアクセス

10.1371/journal.pone.0052383

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引用スタイル

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AU - Zhou, Yue

AU - Sakurai, Hiroaki

AU - Andoh, Tsugunobu

AU - Jin, Aishun

AU - Kishi, Hiroyuki

AU - Muraguchi, Atsushi

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