抄録
The modular architecture of naturally occurring ribozymes makes them a promising class of structural platform for the design and assembly of three-dimensional (3D) RNA nanostructures, into which the catalytic ability of the platform ribozyme can be installed. We have constructed and analyzed RNA nanostructures with polygonal-shaped (closed) ribozyme oligomers by assembling unit RNAs derived from the Tetrahymena group I intron with a typical modular architecture. In this study, we dimerized ribozyme trimers with a triangular shape by introducing three pillar units. The resulting double-decker nanostructures containing six ribozyme units were characterized biochemically and their structures were observed by atomic force microscopy. The double-decker hexamers exhibited higher catalytic activity than the parent ribozyme trimers.
本文言語 | 英語 |
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論文番号 | e202100573 |
ジャーナル | ChemBioChem |
巻 | 23 |
号 | 6 |
DOI | |
出版ステータス | 出版済み - 2022/03/18 |
ASJC Scopus 主題領域
- 生化学
- 分子医療
- 分子生物学
- 有機化学