Segmental isotope-labeling of the intrinsically disordered protein PQBP1

Yuko Nabeshima; Mineyuki Mizuguchi; Asagi Kajiyama; Hitoshi Okazawa

doi:10.1016/j.febslet.2014.10.028

Segmental isotope-labeling of the intrinsically disordered protein PQBP1

Yuko Nabeshima, Mineyuki Mizuguchi^*, Asagi Kajiyama, Hitoshi Okazawa

^*Corresponding author for this work

Structural Biology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced a segmentally isotope-labeled PQBP1 composed of a non-labeled segment (residues 1-219; N-segment) and a ¹³C/¹⁵N-labeled segment (residues 220-265; C-segment). Our results demonstrate that the segmental isotope-labeling combined with NMR spectroscopy is useful for detecting a very weak intra-molecular interaction in an intrinsically disordered protein.

Original language	English
Pages (from-to)	4583-4589
Number of pages	7
Journal	FEBS Letters
Volume	588
Issue number	24
DOIs	https://doi.org/10.1016/j.febslet.2014.10.028
State	Published - 2014/12/20

Keywords

Expressed protein ligation
Intra-molecular interaction
Intrinsically disordered protein
NMR
Segmental isotope-labeling

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Segmental isotope-labeling of the intrinsically disordered protein PQBP1",

abstract = "Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced a segmentally isotope-labeled PQBP1 composed of a non-labeled segment (residues 1-219; N-segment) and a 13C/15N-labeled segment (residues 220-265; C-segment). Our results demonstrate that the segmental isotope-labeling combined with NMR spectroscopy is useful for detecting a very weak intra-molecular interaction in an intrinsically disordered protein.",

keywords = "Expressed protein ligation, Intra-molecular interaction, Intrinsically disordered protein, NMR, Segmental isotope-labeling",

author = "Yuko Nabeshima and Mineyuki Mizuguchi and Asagi Kajiyama and Hitoshi Okazawa",

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T1 - Segmental isotope-labeling of the intrinsically disordered protein PQBP1

AU - Nabeshima, Yuko

AU - Mizuguchi, Mineyuki

AU - Kajiyama, Asagi

AU - Okazawa, Hitoshi

PY - 2014/12/20

Y1 - 2014/12/20

N2 - Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced a segmentally isotope-labeled PQBP1 composed of a non-labeled segment (residues 1-219; N-segment) and a 13C/15N-labeled segment (residues 220-265; C-segment). Our results demonstrate that the segmental isotope-labeling combined with NMR spectroscopy is useful for detecting a very weak intra-molecular interaction in an intrinsically disordered protein.

AB - Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced a segmentally isotope-labeled PQBP1 composed of a non-labeled segment (residues 1-219; N-segment) and a 13C/15N-labeled segment (residues 220-265; C-segment). Our results demonstrate that the segmental isotope-labeling combined with NMR spectroscopy is useful for detecting a very weak intra-molecular interaction in an intrinsically disordered protein.

KW - Expressed protein ligation

KW - Intra-molecular interaction

KW - Intrinsically disordered protein

KW - NMR

KW - Segmental isotope-labeling

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U2 - 10.1016/j.febslet.2014.10.028

DO - 10.1016/j.febslet.2014.10.028

M3 - 学術論文

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SN - 0014-5793

VL - 588

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EP - 4589

JO - FEBS Letters

JF - FEBS Letters

IS - 24

ER -

Segmental isotope-labeling of the intrinsically disordered protein PQBP1

Abstract

Keywords

ASJC Scopus subject areas

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