Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase

Michiko Jo; Norio Nakamura; Nobuko Kakiuchi; Katsuko Komatsu; Ming Hua Qui; Kumiko Shimotohno; Kunitada Shimotohno; Masao Hattori

doi:10.1007/s11418-006-0041-7

Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase

Michiko Jo, Norio Nakamura, Nobuko Kakiuchi, Katsuko Komatsu, Ming Hua Qui, Kumiko Shimotohno, Kunitada Shimotohno, Masao Hattori^*

^*Corresponding author for this work

Department of Medicinal Resources Management, Institute of Natural Medicine
Section of Pharmacognosy, Division of Medicinal Resources, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

For the purpose of developing novel anti-hepatitis C virus (HCV) agents from natural resources, 93 Yunnan crude drugs were screened for their inhibitory effects on RNA-dependent RNA polymerase (RdRp) of HCV. Although 71 methanol extracts and 50 water extracts inhibited HCV-RdRp by more than 50% at a concentration of 50 μg/ml, the majority of them contained a high percentage of tannins. However, methanol extracts of Plumbago zeylanica (branch), Maytenus fookerii (leaf) and Huashidancha (Y61, branch and leaf), and water extracts of Potentilla griffithii (whole plant) and Salvia yunnanensis (underground part), having IC₅₀ values of less than 10 μg/ml, showed less than 10% tannin content. In addition, from a methanol extract of Tripterygium hypoglaucum (root bark), demethylzeylasteral was isolated as a strongly inhibitory substance against HCV-RdRp.

Original language	English
Pages (from-to)	217-224
Number of pages	8
Journal	Natural Medicines
Volume	60
Issue number	3
DOIs	https://doi.org/10.1007/s11418-006-0041-7
State	Published - 2006/07

Keywords

Demethylzeylasteral
Hepatitis C virus
RNA-dependent RNA polymerase
Tripterygium hypoglaucum
Yunnan crude drugs

ASJC Scopus subject areas

Molecular Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11418-006-0041-7

Cite this

@article{e9d75270b3234535b3823f04fd58171d,

title = "Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase",

abstract = "For the purpose of developing novel anti-hepatitis C virus (HCV) agents from natural resources, 93 Yunnan crude drugs were screened for their inhibitory effects on RNA-dependent RNA polymerase (RdRp) of HCV. Although 71 methanol extracts and 50 water extracts inhibited HCV-RdRp by more than 50% at a concentration of 50 μg/ml, the majority of them contained a high percentage of tannins. However, methanol extracts of Plumbago zeylanica (branch), Maytenus fookerii (leaf) and Huashidancha (Y61, branch and leaf), and water extracts of Potentilla griffithii (whole plant) and Salvia yunnanensis (underground part), having IC50 values of less than 10 μg/ml, showed less than 10% tannin content. In addition, from a methanol extract of Tripterygium hypoglaucum (root bark), demethylzeylasteral was isolated as a strongly inhibitory substance against HCV-RdRp.",

keywords = "Demethylzeylasteral, Hepatitis C virus, RNA-dependent RNA polymerase, Tripterygium hypoglaucum, Yunnan crude drugs",

author = "Michiko Jo and Norio Nakamura and Nobuko Kakiuchi and Katsuko Komatsu and Qui, {Ming Hua} and Kumiko Shimotohno and Kunitada Shimotohno and Masao Hattori",

note = "Funding Information: Medicinal plants (crude drugs) used in this study were collected in Yunnan Province of China in 2000, during the Japan Society for the Promotion of Science (JSPS) Project of Overseas Survey on Ethnical Medicines [12– 15]. The root barks of T. hypoglaucum were obtained from Kunming, Yunnan Province, China, and the botanical source was identified by K.K. These specimens are deposited in the Herbarium of Materia Medica of University of Toyama (TMPW no. 24248, 24249).",

year = "2006",

month = jul,

doi = "10.1007/s11418-006-0041-7",

language = "英語",

volume = "60",

pages = "217--224",

journal = "Natural Medicines",

issn = "1340-3443",

publisher = "Springer Japan",

number = "3",

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TY - JOUR

T1 - Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase

AU - Jo, Michiko

AU - Nakamura, Norio

AU - Kakiuchi, Nobuko

AU - Komatsu, Katsuko

AU - Qui, Ming Hua

AU - Shimotohno, Kumiko

AU - Shimotohno, Kunitada

AU - Hattori, Masao

N1 - Funding Information: Medicinal plants (crude drugs) used in this study were collected in Yunnan Province of China in 2000, during the Japan Society for the Promotion of Science (JSPS) Project of Overseas Survey on Ethnical Medicines [12– 15]. The root barks of T. hypoglaucum were obtained from Kunming, Yunnan Province, China, and the botanical source was identified by K.K. These specimens are deposited in the Herbarium of Materia Medica of University of Toyama (TMPW no. 24248, 24249).

PY - 2006/7

Y1 - 2006/7

N2 - For the purpose of developing novel anti-hepatitis C virus (HCV) agents from natural resources, 93 Yunnan crude drugs were screened for their inhibitory effects on RNA-dependent RNA polymerase (RdRp) of HCV. Although 71 methanol extracts and 50 water extracts inhibited HCV-RdRp by more than 50% at a concentration of 50 μg/ml, the majority of them contained a high percentage of tannins. However, methanol extracts of Plumbago zeylanica (branch), Maytenus fookerii (leaf) and Huashidancha (Y61, branch and leaf), and water extracts of Potentilla griffithii (whole plant) and Salvia yunnanensis (underground part), having IC50 values of less than 10 μg/ml, showed less than 10% tannin content. In addition, from a methanol extract of Tripterygium hypoglaucum (root bark), demethylzeylasteral was isolated as a strongly inhibitory substance against HCV-RdRp.

AB - For the purpose of developing novel anti-hepatitis C virus (HCV) agents from natural resources, 93 Yunnan crude drugs were screened for their inhibitory effects on RNA-dependent RNA polymerase (RdRp) of HCV. Although 71 methanol extracts and 50 water extracts inhibited HCV-RdRp by more than 50% at a concentration of 50 μg/ml, the majority of them contained a high percentage of tannins. However, methanol extracts of Plumbago zeylanica (branch), Maytenus fookerii (leaf) and Huashidancha (Y61, branch and leaf), and water extracts of Potentilla griffithii (whole plant) and Salvia yunnanensis (underground part), having IC50 values of less than 10 μg/ml, showed less than 10% tannin content. In addition, from a methanol extract of Tripterygium hypoglaucum (root bark), demethylzeylasteral was isolated as a strongly inhibitory substance against HCV-RdRp.

KW - Demethylzeylasteral

KW - Hepatitis C virus

KW - RNA-dependent RNA polymerase

KW - Tripterygium hypoglaucum

KW - Yunnan crude drugs

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SN - 1340-3443

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JO - Natural Medicines

JF - Natural Medicines

IS - 3

ER -

Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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