Differential effects of DNA tumor virus nuclear oncogene products on adipocyte differentiation

Kazuyuki Tobe; Masato Kasuga; Hidero Kitasato; Fumimaro Takaku; Toshiya Takano; Kaoru Segawa

doi:10.1016/0014-5793(87)80175-8

Differential effects of DNA tumor virus nuclear oncogene products on adipocyte differentiation

Kazuyuki Tobe, Masato Kasuga, Hidero Kitasato, Fumimaro Takaku, Toshiya Takano, Kaoru Segawa^*

^*Corresponding author for this work

Internal Medicine （1）

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We have introduced SV40 and polyoma large T antigen- and adenovirus-type 12 E1A genes into mouse 3T3-L1 preadipocyte cells to study the ability of various nuclear oncogene products to modulate cell differentiation. Clones expressing E1A products could differentiate into adipocytes faster than the control in spite of the absence of adipogenic inducers, as measured by the appearance of lipid droplets microscopically and by staining accumulated triglycerides with oil red O. However, clones expressing SV40 and polyoma large T antigens could not differentiate even if they were exposed to the inducers.

Original language	English
Pages (from-to)	345-349
Number of pages	5
Journal	FEBS Letters
Volume	215
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(87)80175-8
State	Published - 1987/05/11

Keywords

Adipogenic differentiation
DNA tumor virus
Nuclear oncogene

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Differential effects of DNA tumor virus nuclear oncogene products on adipocyte differentiation",

abstract = "We have introduced SV40 and polyoma large T antigen- and adenovirus-type 12 E1A genes into mouse 3T3-L1 preadipocyte cells to study the ability of various nuclear oncogene products to modulate cell differentiation. Clones expressing E1A products could differentiate into adipocytes faster than the control in spite of the absence of adipogenic inducers, as measured by the appearance of lipid droplets microscopically and by staining accumulated triglycerides with oil red O. However, clones expressing SV40 and polyoma large T antigens could not differentiate even if they were exposed to the inducers.",

keywords = "Adipogenic differentiation, DNA tumor virus, Nuclear oncogene",

author = "Kazuyuki Tobe and Masato Kasuga and Hidero Kitasato and Fumimaro Takaku and Toshiya Takano and Kaoru Segawa",

year = "1987",

month = may,

day = "11",

doi = "10.1016/0014-5793(87)80175-8",

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T1 - Differential effects of DNA tumor virus nuclear oncogene products on adipocyte differentiation

AU - Tobe, Kazuyuki

AU - Kasuga, Masato

AU - Kitasato, Hidero

AU - Takaku, Fumimaro

AU - Takano, Toshiya

AU - Segawa, Kaoru

PY - 1987/5/11

Y1 - 1987/5/11

N2 - We have introduced SV40 and polyoma large T antigen- and adenovirus-type 12 E1A genes into mouse 3T3-L1 preadipocyte cells to study the ability of various nuclear oncogene products to modulate cell differentiation. Clones expressing E1A products could differentiate into adipocytes faster than the control in spite of the absence of adipogenic inducers, as measured by the appearance of lipid droplets microscopically and by staining accumulated triglycerides with oil red O. However, clones expressing SV40 and polyoma large T antigens could not differentiate even if they were exposed to the inducers.

AB - We have introduced SV40 and polyoma large T antigen- and adenovirus-type 12 E1A genes into mouse 3T3-L1 preadipocyte cells to study the ability of various nuclear oncogene products to modulate cell differentiation. Clones expressing E1A products could differentiate into adipocytes faster than the control in spite of the absence of adipogenic inducers, as measured by the appearance of lipid droplets microscopically and by staining accumulated triglycerides with oil red O. However, clones expressing SV40 and polyoma large T antigens could not differentiate even if they were exposed to the inducers.

KW - Adipogenic differentiation

KW - DNA tumor virus

KW - Nuclear oncogene

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SN - 0014-5793

VL - 215

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EP - 349

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Differential effects of DNA tumor virus nuclear oncogene products on adipocyte differentiation

Abstract

Keywords

ASJC Scopus subject areas

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