Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

Hiroyuki Morita; Takahiro Mori; Kiyofumi Wanibuchi; Ryohei Kato; Shigetoshi Sugio; Ikuro Abe

doi:10.1107/S1744309111002181

Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

Hiroyuki Morita, Takahiro Mori, Kiyofumi Wanibuchi, Ryohei Kato, Shigetoshi Sugio^*, Ikuro Abe

^*Corresponding author for this work

Section of Natural Products & Drug Discovery, Division of Medicinal Resources, Institute of Natural Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2₁, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 Å,α = γ = 90.0, β = 111.1°.

Original language	English
Pages (from-to)	409-411
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	67
Issue number	3
DOIs	https://doi.org/10.1107/S1744309111002181
State	Published - 2011/03

Keywords

ANL superfamily
CoA ligases
lignin biosynthesis
phenylpropanoids

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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title = "Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana",

abstract = "4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 {\AA},α = γ = 90.0, β = 111.1°.",

keywords = "ANL superfamily, CoA ligases, lignin biosynthesis, phenylpropanoids",

author = "Hiroyuki Morita and Takahiro Mori and Kiyofumi Wanibuchi and Ryohei Kato and Shigetoshi Sugio and Ikuro Abe",

year = "2011",

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doi = "10.1107/S1744309111002181",

language = "英語",

volume = "67",

pages = "409--411",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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T1 - Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

AU - Morita, Hiroyuki

AU - Mori, Takahiro

AU - Wanibuchi, Kiyofumi

AU - Kato, Ryohei

AU - Sugio, Shigetoshi

AU - Abe, Ikuro

PY - 2011/3

Y1 - 2011/3

N2 - 4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 Å,α = γ = 90.0, β = 111.1°.

AB - 4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 Å,α = γ = 90.0, β = 111.1°.

KW - ANL superfamily

KW - CoA ligases

KW - lignin biosynthesis

KW - phenylpropanoids

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DO - 10.1107/S1744309111002181

M3 - 学術論文

C2 - 21393854

AN - SCOPUS:79952669657

SN - 1744-3091

VL - 67

SP - 409

EP - 411

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 3

ER -

Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

Abstract

Keywords

ASJC Scopus subject areas

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