Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

Hiroyuki Morita; Takahiro Mori; Kiyofumi Wanibuchi; Ryohei Kato; Shigetoshi Sugio; Ikuro Abe

doi:10.1107/S1744309111002181

Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

Hiroyuki Morita, Takahiro Mori, Kiyofumi Wanibuchi, Ryohei Kato, Shigetoshi Sugio^*, Ikuro Abe

^*この論文の責任著者

和漢医薬学総合研究所研究開発部門資源開発分野（天然物創薬学領域）

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

5 被引用数 (Scopus)

抄録

4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P2₁, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 Å,α = γ = 90.0, β = 111.1°.

本文言語	英語
ページ（範囲）	409-411
ページ数	3
ジャーナル	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
巻	67
号	3
DOI	https://doi.org/10.1107/S1744309111002181
出版ステータス	出版済み - 2011/03

ASJC Scopus 主題領域

生物理学
構造生物学
生化学
遺伝学
凝縮系物理学

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10.1107/S1744309111002181

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title = "Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana",

abstract = "4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 {\AA},α = γ = 90.0, β = 111.1°.",

keywords = "ANL superfamily, CoA ligases, lignin biosynthesis, phenylpropanoids",

author = "Hiroyuki Morita and Takahiro Mori and Kiyofumi Wanibuchi and Ryohei Kato and Shigetoshi Sugio and Ikuro Abe",

year = "2011",

month = mar,

doi = "10.1107/S1744309111002181",

language = "英語",

volume = "67",

pages = "409--411",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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T1 - Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

AU - Morita, Hiroyuki

AU - Mori, Takahiro

AU - Wanibuchi, Kiyofumi

AU - Kato, Ryohei

AU - Sugio, Shigetoshi

AU - Abe, Ikuro

PY - 2011/3

Y1 - 2011/3

N2 - 4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 Å,α = γ = 90.0, β = 111.1°.

AB - 4-Coumarate:CoA ligase 2 (4CL2) from Arabidopsis thaliana catalyzes the ATP-dependent formation of the 4-coumaroyl-CoA thioester through the formation of 4-coumarate-AMP. Recombinant 4CL2 protein was expressed in Escherichia coli and crystallized by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 91.6, b = 55.5, c = 124.4 Å,α = γ = 90.0, β = 111.1°.

KW - ANL superfamily

KW - CoA ligases

KW - lignin biosynthesis

KW - phenylpropanoids

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DO - 10.1107/S1744309111002181

M3 - 学術論文

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AN - SCOPUS:79952669657

SN - 1744-3091

VL - 67

SP - 409

EP - 411

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 3

ER -

Crystallization and preliminary X-ray analysis of 4 - Coumarate:CoA ligase from Arabidopsis thaliana

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