Elucidation of Molecular Mechanism of Cell Death induced by Radiation, Hyperthermia and Ultrasound for Cancer Therapy

  • 近藤, 隆 (Principal Investigator)
  • 田渕, 圭章 (Co-Investigator(Kenkyū-buntansha))
  • 趙, 慶利 (Co-Investigator(Kenkyū-buntansha))
  • TAKASAKI, Ichro (Co-Investigator(Kenkyū-buntansha))

Project Details

Abstract

Molecular mechanism of cell death induced by ionizing radiation, heat or ultrasound was examined. Stable knockdown of TAK1 caused increase in subG1 fraction and apoptosis induced by X-rays in HeLa cells. Inhibition of DNA-PK increased heat-induced apoptosis in HeLa cells and the knockdown of an identified gene, NR1D1, also enhanced apoptosis. For the cell death induced by ultrasound, cell death signal is modulated by the balance between p53 and Akt via ATM and DNA-PK and the pathway of DNA-PK/Akt is predominant in sonicated cells.
StatusFinished
Effective start/end date2010/01/012012/12/31

Funding

  • Japan Society for the Promotion of Science: ¥13,260,000.00

Keywords

  • 放射線腫瘍学
  • 超音波治療学
  • 温熱治療学
  • ハイパーサーミア
  • アポトーシス
  • 放射線
  • 超音波
  • TAK1
  • Chk1
  • 遺伝子発現
  • がん
  • 細胞死
  • 治療