Voltammetric Sensing of Soybean Agglutinin Using an Electrode Modified with Electron-transfer, Carbohydrate-mimetic/Cross-linker-peptide-collagen Film

We constructed an electrochemical sensor based on an electrode modified with electron-transfer, carbohydrate-mimetic peptides on collagen film. The peptide consisted of Ac-Y₄C combined with soybean agglutinin (SBA). To evaluate the binding between SBA and the peptide, we prepared Ac-Y₄CA_n (3-6) containing oligoalanine as a cross-linker. When SBA and Y₄C on the electrode were incubated, the peak was decreased by the SBA uptake of the peptide. The change in the peak current using Ac-Y₄CA₆ was the greatest of the four peptides. The calibration curve was linear and ranged from 4.0×10⁻¹⁴ to 1.2×10⁻¹² M with a detection limit of 1.3×10⁻¹⁴ M.