TY - JOUR
T1 - Synaptopodin maintains the neural activity-dependent enlargement of dendritic spines in hippocampal neurons
AU - Okubo-Suzuki, Reiko
AU - Okada, Daisuke
AU - Sekiguchi, Mariko
AU - Inokuchi, Kaoru
N1 - Funding Information:
We would like to thank Dr. Y. Saitoh for providing the LTP-induced rat brains for in situ hybridization experiments, Dr. Y. Shoji-Kasai for providing the low density cultures of hippocampal neurons, Ms. F. Ozawa for her instruction on culture methods, and Dr. N. Ohkawa for advice on in situ hybridization experiments. This work was supported by the Special Coordinate Funds for Promoting Science and Technology from MEXT of the Japanese Government (K.I.), and supported in part by Grants-in-Aid for Scientific Research on Priority Areas , , and , from MEXT of the Japanese Government (K.I.).
PY - 2008/6
Y1 - 2008/6
N2 - Synaptopodin (SYNPO) is an F-actin interacting protein expressed in dendritic spines and upregulated during the late-phase of long-term potentiation. Here, we investigated whether SYNPO regulates spine morphology through interactions with F-actin, the major cytoskeletal element of spines. In primary hippocampal neuron cultures, both endogenous and exogenous SYNPO localized preferentially in large spines under basal conditions. SYNPO overexpression did not affect the number or volume of spines in unstimulated neurons. Pharmacological activation of synaptic NMDA receptors transiently increased spine volume in control neurons, while the increase was persistent in neurons overexpressing SYNPO. In addition, exogenous SYNPO in PtK2 cells suppressed staurosporine-dependent disruption of F-actin stress fibers, suggesting that SYNPO protected F-actin from disruption. These results suggest that SYNPO stabilized activity-dependent increases in spine volume and imply that late-phase changes in spine morphology involve SYNPO.
AB - Synaptopodin (SYNPO) is an F-actin interacting protein expressed in dendritic spines and upregulated during the late-phase of long-term potentiation. Here, we investigated whether SYNPO regulates spine morphology through interactions with F-actin, the major cytoskeletal element of spines. In primary hippocampal neuron cultures, both endogenous and exogenous SYNPO localized preferentially in large spines under basal conditions. SYNPO overexpression did not affect the number or volume of spines in unstimulated neurons. Pharmacological activation of synaptic NMDA receptors transiently increased spine volume in control neurons, while the increase was persistent in neurons overexpressing SYNPO. In addition, exogenous SYNPO in PtK2 cells suppressed staurosporine-dependent disruption of F-actin stress fibers, suggesting that SYNPO protected F-actin from disruption. These results suggest that SYNPO stabilized activity-dependent increases in spine volume and imply that late-phase changes in spine morphology involve SYNPO.
UR - http://www.scopus.com/inward/record.url?scp=44649119762&partnerID=8YFLogxK
U2 - 10.1016/j.mcn.2008.03.001
DO - 10.1016/j.mcn.2008.03.001
M3 - 学術論文
C2 - 18424168
AN - SCOPUS:44649119762
SN - 1044-7431
VL - 38
SP - 266
EP - 276
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 2
ER -