TY - CHAP
T1 - Screening of antigen-specific antibody-secreting cells
AU - Kishi, Hiroyuki
AU - Jin, Aishun
AU - Ozawa, Tatsuhiko
AU - Tajiri, Kazuto
AU - Obata, Tsutomu
AU - Muraguchi, Atsushi
PY - 2012
Y1 - 2012
N2 - Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific monoclonal antibodies. In human system, good hybridoma-producing system is not available. Instead, transformation of B-lymphocytes with Epstein-Barr viruses is used to obtain antibody-secreting cell lines. Furthermore, phage-display system using molecular biology is recently used to obtain antigen-specific human monoclonal antibodies. Here, we describe the new method for screening antigen-specific antibody-secreting cells at single-cell levels using microwell-array chips. The system can be applied to screen antigen-specific antibody-secreting cells from any animal species.
AB - Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific monoclonal antibodies. In human system, good hybridoma-producing system is not available. Instead, transformation of B-lymphocytes with Epstein-Barr viruses is used to obtain antibody-secreting cell lines. Furthermore, phage-display system using molecular biology is recently used to obtain antigen-specific human monoclonal antibodies. Here, we describe the new method for screening antigen-specific antibody-secreting cells at single-cell levels using microwell-array chips. The system can be applied to screen antigen-specific antibody-secreting cells from any animal species.
KW - Antibody-secreting cell
KW - Antigen-specific antibody
KW - Immunospot array assay on chip
KW - Microwell-array chip
UR - http://www.scopus.com/inward/record.url?scp=84858410511&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-567-1_11
DO - 10.1007/978-1-61779-567-1_11
M3 - 章
C2 - 22323145
AN - SCOPUS:84858410511
SN - 9781617795664
T3 - Methods in Molecular Biology
SP - 141
EP - 150
BT - Single-Cell Analysis
A2 - Lindstrom, Sara
A2 - Andersson-Svahn, Helene
ER -