Screening of antigen-specific antibody-secreting cells

Hiroyuki Kishi; Aishun Jin; Tatsuhiko Ozawa; Kazuto Tajiri; Tsutomu Obata; Atsushi Muraguchi

doi:10.1007/978-1-61779-567-1_11

Screening of antigen-specific antibody-secreting cells

Hiroyuki Kishi^*, Aishun Jin, Tatsuhiko Ozawa, Kazuto Tajiri, Tsutomu Obata, Atsushi Muraguchi

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

3 Scopus citations

Abstract

Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific monoclonal antibodies. In human system, good hybridoma-producing system is not available. Instead, transformation of B-lymphocytes with Epstein-Barr viruses is used to obtain antibody-secreting cell lines. Furthermore, phage-display system using molecular biology is recently used to obtain antigen-specific human monoclonal antibodies. Here, we describe the new method for screening antigen-specific antibody-secreting cells at single-cell levels using microwell-array chips. The system can be applied to screen antigen-specific antibody-secreting cells from any animal species.

Original language	English
Title of host publication	Single-Cell Analysis
Subtitle of host publication	Methods and Protocols
Editors	Sara Lindstrom, Helene Andersson-Svahn
Pages	141-150
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-61779-567-1_11
State	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	853
ISSN (Print)	1064-3745

Keywords

Antibody-secreting cell
Antigen-specific antibody
Immunospot array assay on chip
Microwell-array chip

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-61779-567-1_11

Cite this

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abstract = "Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific monoclonal antibodies. In human system, good hybridoma-producing system is not available. Instead, transformation of B-lymphocytes with Epstein-Barr viruses is used to obtain antibody-secreting cell lines. Furthermore, phage-display system using molecular biology is recently used to obtain antigen-specific human monoclonal antibodies. Here, we describe the new method for screening antigen-specific antibody-secreting cells at single-cell levels using microwell-array chips. The system can be applied to screen antigen-specific antibody-secreting cells from any animal species.",

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AU - Jin, Aishun

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AU - Tajiri, Kazuto

AU - Obata, Tsutomu

AU - Muraguchi, Atsushi

PY - 2012

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KW - Immunospot array assay on chip

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Screening of antigen-specific antibody-secreting cells

Abstract

Publication series

Keywords

ASJC Scopus subject areas

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