抄録
It is commonly understood that T cells are activated via trans interactions between antigen-specific T-cell receptors (TCRs) and antigenic peptides presented on major histocompatibility complex (MHC) molecules on antigen-presenting cells. By analysing a large number of T cells at the single-cell level on a microwell array, we show that T-cell activation can occur via cis interactions (where TCRs on the T cell interact with the antigenic peptides presented on MHC class-I molecules on the same cell), and that such cis activation can be used to detect antigen-specific T cells and clone their TCR within 4 d. We used the detection-and-cloning system to clone a tumour-antigen-specific TCR from peripheral blood mononuclear cells of healthy donors. TCR cloning by leveraging the cis activation of T cells may facilitate the development of TCR-engineered T cells for cancer therapy.
本文言語 | 英語 |
---|---|
ページ(範囲) | 806-818 |
ページ数 | 13 |
ジャーナル | Nature Biomedical Engineering |
巻 | 6 |
号 | 7 |
DOI | |
出版ステータス | 出版済み - 2022/07 |
ASJC Scopus 主題領域
- バイオテクノロジー
- バイオエンジニアリング
- 医学(その他)
- 生体医工学
- コンピュータ サイエンスの応用