Rapid cloning of antigen-specific T-cell receptors by leveraging the cis activation of T cells

Eiji Kobayashi, Aishun Jin, Hiroshi Hamana, Kiyomi Shitaoka, Kazuto Tajiri, Seisuke Kusano, Shigeyuki Yokoyama, Tatsuhiko Ozawa, Tsutomu Obata, Atsushi Muraguchi, Hiroyuki Kishi*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

10 被引用数 (Scopus)

抄録

It is commonly understood that T cells are activated via trans interactions between antigen-specific T-cell receptors (TCRs) and antigenic peptides presented on major histocompatibility complex (MHC) molecules on antigen-presenting cells. By analysing a large number of T cells at the single-cell level on a microwell array, we show that T-cell activation can occur via cis interactions (where TCRs on the T cell interact with the antigenic peptides presented on MHC class-I molecules on the same cell), and that such cis activation can be used to detect antigen-specific T cells and clone their TCR within 4 d. We used the detection-and-cloning system to clone a tumour-antigen-specific TCR from peripheral blood mononuclear cells of healthy donors. TCR cloning by leveraging the cis activation of T cells may facilitate the development of TCR-engineered T cells for cancer therapy.

本文言語英語
ページ(範囲)806-818
ページ数13
ジャーナルNature Biomedical Engineering
6
7
DOI
出版ステータス出版済み - 2022/07

ASJC Scopus 主題領域

  • バイオテクノロジー
  • バイオエンジニアリング
  • 医学(その他)
  • 生体医工学
  • コンピュータ サイエンスの応用

フィンガープリント

「Rapid cloning of antigen-specific T-cell receptors by leveraging the cis activation of T cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル