Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

Shota Morimoto; Takenori Tomohiro; Nobuyuki Maruyama; Yasumaru Hatanaka

doi:10.1039/c3cc38594a

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

Shota Morimoto, Takenori Tomohiro^*, Nobuyuki Maruyama, Yasumaru Hatanaka

^*この論文の責任著者

生体認識化学研究室

研究成果: ジャーナルへの寄稿 › 学術論文 › 査読

18 被引用数 (Scopus)

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A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

本文言語	英語
ページ（範囲）	1811-1813
ページ数	3
ジャーナル	Chemical Communications
巻	49
号	18
DOI	https://doi.org/10.1039/c3cc38594a
出版ステータス	出版済み - 2013/02/05

ASJC Scopus 主題領域

電子材料、光学材料、および磁性材料
触媒
セラミックおよび複合材料
化学一般
表面、皮膜および薄膜
金属および合金
材料化学

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10.1039/c3cc38594a

引用スタイル

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abstract = "A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.",

author = "Shota Morimoto and Takenori Tomohiro and Nobuyuki Maruyama and Yasumaru Hatanaka",

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AU - Tomohiro, Takenori

AU - Maruyama, Nobuyuki

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AB - A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

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DO - 10.1039/c3cc38594a

M3 - 学術論文

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Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

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ASJC Scopus 主題領域

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