Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

Shota Morimoto; Takenori Tomohiro; Nobuyuki Maruyama; Yasumaru Hatanaka

doi:10.1039/c3cc38594a

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

Shota Morimoto, Takenori Tomohiro^*, Nobuyuki Maruyama, Yasumaru Hatanaka

^*Corresponding author for this work

Biorecognition Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

Original language	English
Pages (from-to)	1811-1813
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	18
DOIs	https://doi.org/10.1039/c3cc38594a
State	Published - 2013/02/05

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Catalysis
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/c3cc38594a

Cite this

@article{44462303fd5d4e34a341658d07ce63d2,

title = "Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein",

abstract = "A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.",

author = "Shota Morimoto and Takenori Tomohiro and Nobuyuki Maruyama and Yasumaru Hatanaka",

year = "2013",

month = feb,

day = "5",

doi = "10.1039/c3cc38594a",

language = "英語",

volume = "49",

pages = "1811--1813",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "18",

}

TY - JOUR

T1 - Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

AU - Morimoto, Shota

AU - Tomohiro, Takenori

AU - Maruyama, Nobuyuki

AU - Hatanaka, Yasumaru

PY - 2013/2/5

Y1 - 2013/2/5

N2 - A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

AB - A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

UR - http://www.scopus.com/inward/record.url?scp=84873581398&partnerID=8YFLogxK

U2 - 10.1039/c3cc38594a

DO - 10.1039/c3cc38594a

M3 - 学術論文

C2 - 23349004

AN - SCOPUS:84873581398

SN - 1359-7345

VL - 49

SP - 1811

EP - 1813

JO - Chemical Communications

JF - Chemical Communications

IS - 18

ER -

Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

Abstract

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this