N-acetyltransferase ARD1-NAT1 regulates neuronal dendritic development

Noriaki Ohkawa, Shunichiro Sugisaki, Eri Tokunaga, Kazuko Fujitani, Takahiro Hayasaka, Mitsutoshi Setou, Kaoru Inokuchi*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

81 被引用数 (Scopus)

抄録

ARD1 and NAT1 constitute an N-acetyltransferase complex where ARD1 holds the enzymatic activity of the complex. The ARD1-NAT1 complex mediates N-terminal acetylation of nascent polypeptides that emerge from ribosomes after translation. ARD1 may also acetylate the internal lysine residues of proteins. Although ARD1 and NAT1 have been found in the brain, the physiological role and substrates of the ARD1-NAT1 complex in neurons remain unclear. Here we investigated role of N-acetyltransferase activity in the process of neuronal development. Expression of ARD1 and NAT1 increased during dendritic development, and both proteins colocalized with microtubules in dendrites. The ARD1-NAT1 complex displayed acetyltransferase activity against a purified microtubule fraction in vitro. Inhibition of the complex limited the dendritic extension of cultured neurons. These findings suggest that the ARD1-NAT1 complex has acetyltransferase activity against microtubules in dendrites. Regulation by acetyltransferase activity is a novel mechanism that is required for dendritic arborization during neuronal development.

本文言語英語
ページ(範囲)1171-1183
ページ数13
ジャーナルGenes to Cells
13
11
DOI
出版ステータス出版済み - 2008

ASJC Scopus 主題領域

  • 遺伝学
  • 細胞生物学

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