Laser-scanning flow cytometer with a three-dimensional microfluidic chip

Shingo Imanishi, Motohiro Furuki, Masataka Shinoda, Yohei Morita, Yuji Yamazaki, Hiromitsu Nakauchi

研究成果: 会議への寄与学会論文査読

抄録

We have developed a new flow cytometer utilizing a three-dimensional (3D) microfluidic disposable plastic chip and real-time multi-spectral deconvolution analysis. The chip has microfluidic channels where sample particles flow down in the center of each channel. Three laser diodes (LDs) are used, of which the laser beams are scanned with a polygonal mirror, and only the centers of the microfluidic channels are irradiated with pulsed flashes. Emitted fluorescence light is separated in spectral distribution with a grating and is detected with a 32-arrayed photomultiplier tube (PMT). The spectral signal is deconvoluted with reference spectra of all fluorescent components.

本文言語英語
ページ305-307
ページ数3
出版ステータス出版済み - 2008
イベント12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, 米国
継続期間: 2008/10/122008/10/16

学会

学会12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
国/地域米国
CitySan Diego, CA
Period2008/10/122008/10/16

ASJC Scopus 主題領域

  • 化学工学(その他)
  • バイオエンジニアリング

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