Laser-scanning flow cytometer with a three-dimensional microfluidic chip

Shingo Imanishi; Motohiro Furuki; Masataka Shinoda; Yohei Morita; Yuji Yamazaki; Hiromitsu Nakauchi

Laser-scanning flow cytometer with a three-dimensional microfluidic chip

Shingo Imanishi, Motohiro Furuki, Masataka Shinoda, Yohei Morita, Yuji Yamazaki, Hiromitsu Nakauchi

Program of Biological Science

Research output: Contribution to conference › Paper › peer-review

Abstract

We have developed a new flow cytometer utilizing a three-dimensional (3D) microfluidic disposable plastic chip and real-time multi-spectral deconvolution analysis. The chip has microfluidic channels where sample particles flow down in the center of each channel. Three laser diodes (LDs) are used, of which the laser beams are scanned with a polygonal mirror, and only the centers of the microfluidic channels are irradiated with pulsed flashes. Emitted fluorescence light is separated in spectral distribution with a grating and is detected with a 32-arrayed photomultiplier tube (PMT). The spectral signal is deconvoluted with reference spectra of all fluorescent components.

Original language	English
Pages	305-307
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008/10/12 → 2008/10/16

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	2008/10/12 → 2008/10/16

Keywords

Microfluidic chip
Scanning flow cytometer
Spectral deconvolution
Three-dimensional laminar flow

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "Laser-scanning flow cytometer with a three-dimensional microfluidic chip",

abstract = "We have developed a new flow cytometer utilizing a three-dimensional (3D) microfluidic disposable plastic chip and real-time multi-spectral deconvolution analysis. The chip has microfluidic channels where sample particles flow down in the center of each channel. Three laser diodes (LDs) are used, of which the laser beams are scanned with a polygonal mirror, and only the centers of the microfluidic channels are irradiated with pulsed flashes. Emitted fluorescence light is separated in spectral distribution with a grating and is detected with a 32-arrayed photomultiplier tube (PMT). The spectral signal is deconvoluted with reference spectra of all fluorescent components.",

keywords = "Microfluidic chip, Scanning flow cytometer, Spectral deconvolution, Three-dimensional laminar flow",

author = "Shingo Imanishi and Motohiro Furuki and Masataka Shinoda and Yohei Morita and Yuji Yamazaki and Hiromitsu Nakauchi",

year = "2008",

language = "英語",

pages = "305--307",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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Laser-scanning flow cytometer with a three-dimensional microfluidic chip. / Imanishi, Shingo; Furuki, Motohiro; Shinoda, Masataka et al.
2008. 305-307 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Laser-scanning flow cytometer with a three-dimensional microfluidic chip

AU - Imanishi, Shingo

AU - Furuki, Motohiro

AU - Shinoda, Masataka

AU - Morita, Yohei

AU - Yamazaki, Yuji

AU - Nakauchi, Hiromitsu

PY - 2008

Y1 - 2008

N2 - We have developed a new flow cytometer utilizing a three-dimensional (3D) microfluidic disposable plastic chip and real-time multi-spectral deconvolution analysis. The chip has microfluidic channels where sample particles flow down in the center of each channel. Three laser diodes (LDs) are used, of which the laser beams are scanned with a polygonal mirror, and only the centers of the microfluidic channels are irradiated with pulsed flashes. Emitted fluorescence light is separated in spectral distribution with a grating and is detected with a 32-arrayed photomultiplier tube (PMT). The spectral signal is deconvoluted with reference spectra of all fluorescent components.

AB - We have developed a new flow cytometer utilizing a three-dimensional (3D) microfluidic disposable plastic chip and real-time multi-spectral deconvolution analysis. The chip has microfluidic channels where sample particles flow down in the center of each channel. Three laser diodes (LDs) are used, of which the laser beams are scanned with a polygonal mirror, and only the centers of the microfluidic channels are irradiated with pulsed flashes. Emitted fluorescence light is separated in spectral distribution with a grating and is detected with a 32-arrayed photomultiplier tube (PMT). The spectral signal is deconvoluted with reference spectra of all fluorescent components.

KW - Microfluidic chip

KW - Scanning flow cytometer

KW - Spectral deconvolution

KW - Three-dimensional laminar flow

UR - http://www.scopus.com/inward/record.url?scp=84902492324&partnerID=8YFLogxK

M3 - 学会論文

AN - SCOPUS:84902492324

SP - 305

EP - 307

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Laser-scanning flow cytometer with a three-dimensional microfluidic chip

Abstract

Conference

Keywords

ASJC Scopus subject areas

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