TY - JOUR
T1 - Homer proteins control neuronal differentiation through IP3 receptor signaling
AU - Tanaka, Masahiko
AU - Duncan, R. Scott
AU - McClung, Nathalie
AU - Yannazzo, Jo Ann S.
AU - Hwang, Sung Yong
AU - Marunouchi, Tohru
AU - Inokuchi, Kaoru
AU - Koulen, Peter
N1 - Funding Information:
We thank Dr. J. Miyazaki (Osaka University) for the gift of the CAG promoter, Dr. Y. Kadokawa (Fujita Health University) for the gift of the pCAG-IRES-hrGFP and Dr. T. Kameyama (Fujita Health University) for technical advice on the construction of expression plasmids. This study was in part supported by the Ministry of Education, Culture, Sports, Science and Technology of Japan (MT), Fujita Health University (TM), a Young Investigator Award from The National Alliance for Research on Schizophrenia and Depression (PK) and NIH/NIA Grant AG022550 (PK) and NIH/NCRR grant RR022570 (PK). We thank M. Koulen, R. Koulen, S. Koulen, M. Tanaka, M. Tanaka and E. Tanaka for generous support and encouragement. The authors declare no competing interests. Masahiko Tanaka and Peter Koulen contributed equally to the work.
PY - 2006/11/13
Y1 - 2006/11/13
N2 - Neurons expand, sustain or prune their dendritic trees during ontogenesis [Cline, H.T. (2001). Dendritic arbor development and synaptogenesis. Curr. Opin. Neurobiol. 11, 118-126; Wong, W.T. and Wong, R.O.L. (2000) Rapid dendritic movements during synapse formation and rearrangement. Curr. Opin. Neurobiol. 10, 118-124] which critically depends on neuronal activity [Wong, W.T., Faulkner-Jones, B.E., Sanes, J.R. and Wong, R.O.L. (2000) Rapid dendritic remodeling in the developing retina: dependence on neurotransmission and reciprocal regulation by Rac and Rho. J. Neurosci. 20, 5024-5036; Li, Z., Van Aelst, L. and Cline, H.T. (2000) Rho GTPases regulate distinct aspects of dendritic arbor growth in Xenopus central neurons in vivo. Nat. Neurosci. 3, 217-225; Wong, W.T. and Wong, R.O.L. (2001) Changing specificity of neurotransmitter regulation of rapid dendritic remodeling during synaptogenesis. Nat. Neurosci. 4, 351-352.] and sub-cellular Ca2+ signals [Lohmann, C., Myhr, K.L. and Wong, R.O. (2002) Transmitter-evoked local calcium release stabilizes developing dendrites, Nature 418, 177-181.]. The role of synaptic clustering proteins connecting both processes is unclear. Here, we show that expression levels of Vesl-1/Homer 1 isoforms critically control properties of Ca2+ release from intracellular stores and dendritic morphology of CNS neurons. Vesl-1L/Homer 1c, an isoform with a functional WH1 and coiled-coil domain, but not isoforms missing these features were capable of potentiating intracellular calcium signaling activity indicating that such regulatory interactions function as a general paradigm in cellular differentiation and are subject to changes in expression levels of Vesl/Homer isoforms.
AB - Neurons expand, sustain or prune their dendritic trees during ontogenesis [Cline, H.T. (2001). Dendritic arbor development and synaptogenesis. Curr. Opin. Neurobiol. 11, 118-126; Wong, W.T. and Wong, R.O.L. (2000) Rapid dendritic movements during synapse formation and rearrangement. Curr. Opin. Neurobiol. 10, 118-124] which critically depends on neuronal activity [Wong, W.T., Faulkner-Jones, B.E., Sanes, J.R. and Wong, R.O.L. (2000) Rapid dendritic remodeling in the developing retina: dependence on neurotransmission and reciprocal regulation by Rac and Rho. J. Neurosci. 20, 5024-5036; Li, Z., Van Aelst, L. and Cline, H.T. (2000) Rho GTPases regulate distinct aspects of dendritic arbor growth in Xenopus central neurons in vivo. Nat. Neurosci. 3, 217-225; Wong, W.T. and Wong, R.O.L. (2001) Changing specificity of neurotransmitter regulation of rapid dendritic remodeling during synaptogenesis. Nat. Neurosci. 4, 351-352.] and sub-cellular Ca2+ signals [Lohmann, C., Myhr, K.L. and Wong, R.O. (2002) Transmitter-evoked local calcium release stabilizes developing dendrites, Nature 418, 177-181.]. The role of synaptic clustering proteins connecting both processes is unclear. Here, we show that expression levels of Vesl-1/Homer 1 isoforms critically control properties of Ca2+ release from intracellular stores and dendritic morphology of CNS neurons. Vesl-1L/Homer 1c, an isoform with a functional WH1 and coiled-coil domain, but not isoforms missing these features were capable of potentiating intracellular calcium signaling activity indicating that such regulatory interactions function as a general paradigm in cellular differentiation and are subject to changes in expression levels of Vesl/Homer isoforms.
KW - Calcium
KW - Cerebellum
KW - Dendrite
KW - Neuronal development
KW - Purkinje cell
UR - http://www.scopus.com/inward/record.url?scp=33750588000&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2006.10.013
DO - 10.1016/j.febslet.2006.10.013
M3 - 学術論文
C2 - 17064693
AN - SCOPUS:33750588000
SN - 0014-5793
VL - 580
SP - 6145
EP - 6150
JO - FEBS Letters
JF - FEBS Letters
IS - 26
ER -