@inproceedings{e1babac11b1241549771ad0db77cf39b,
title = "High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy",
abstract = "We used only a narrow range, 1397-1501 cm-1, for high-throughput analysis of cancerous and noncancerous human cell lines by spontaneous Raman microscopy. With baseline-corrected cellwise spectra in this range, two cell lines were discriminated at accuracy higher than 90%. This narrowband measurement allowed reduction of the signal readout time by 24-folds in comparison to a correspondent wideband measurement detecting 536-3132 cm-1, enabling cell analysis at 2.5 cells/min. To further improve the throughput, we employed detector binning, which allowed reduction not just of the readout time but also of the signal accumulation time with maintaining signal-to-background ratio and the accuracy. Improvement of the imaging speed by this approach reached at 4-folds, enabling a high-throughout analysis at 10 cells/min.",
keywords = "Cancer detection, Discriminant analysis, Narrowband, Raman microscopy, Spectral resolution",
author = "Yasuaki Kumamoto and Kentaro Mochizuki and Kosuke Hashimoto and Yoshinori Harada and Hideo Tanaka and Katsumasa Fujita",
note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; 6th Biomedical Imaging and Sensing Conference ; Conference date: 20-04-2020 Through 24-04-2020",
year = "2020",
doi = "10.1117/12.2573223",
language = "英語",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
editor = "Toyohiko Yatagai and Yoshihisa Aizu and Osamu Matoba and Yasuhiro Awatsuji and Yuan Luo",
booktitle = "Biomedical Imaging and Sensing Conference 2020",
}
