High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy

Yasuaki Kumamoto; Kentaro Mochizuki; Kosuke Hashimoto; Yoshinori Harada; Hideo Tanaka; Katsumasa Fujita

doi:10.1117/12.2573223

High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy

Yasuaki Kumamoto, Kentaro Mochizuki, Kosuke Hashimoto, Yoshinori Harada, Hideo Tanaka, Katsumasa Fujita

Research Center for Pre-Disease Science

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We used only a narrow range, 1397-1501 cm^-1, for high-throughput analysis of cancerous and noncancerous human cell lines by spontaneous Raman microscopy. With baseline-corrected cellwise spectra in this range, two cell lines were discriminated at accuracy higher than 90%. This narrowband measurement allowed reduction of the signal readout time by 24-folds in comparison to a correspondent wideband measurement detecting 536-3132 cm^-1, enabling cell analysis at 2.5 cells/min. To further improve the throughput, we employed detector binning, which allowed reduction not just of the readout time but also of the signal accumulation time with maintaining signal-to-background ratio and the accuracy. Improvement of the imaging speed by this approach reached at 4-folds, enabling a high-throughout analysis at 10 cells/min.

Original language	English
Title of host publication	Biomedical Imaging and Sensing Conference 2020
Editors	Toyohiko Yatagai, Yoshihisa Aizu, Osamu Matoba, Yasuhiro Awatsuji, Yuan Luo
Publisher	SPIE
ISBN (Electronic)	9781510638495
DOIs	https://doi.org/10.1117/12.2573223
State	Published - 2020
Event	6th Biomedical Imaging and Sensing Conference - Yokohama, Japan Duration: 2020/04/20 → 2020/04/24

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11521
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	6th Biomedical Imaging and Sensing Conference
Country/Territory	Japan
City	Yokohama
Period	2020/04/20 → 2020/04/24

Keywords

Cancer detection
Discriminant analysis
Narrowband
Raman microscopy
Spectral resolution

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.2573223

Cite this

Kumamoto, Y., Mochizuki, K., Hashimoto, K., Harada, Y., Tanaka, H., & Fujita, K. (2020). High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy. In T. Yatagai, Y. Aizu, O. Matoba, Y. Awatsuji, & Y. Luo (Eds.), Biomedical Imaging and Sensing Conference 2020 Article 1152112 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11521). SPIE. https://doi.org/10.1117/12.2573223

Kumamoto, Yasuaki ; Mochizuki, Kentaro ; Hashimoto, Kosuke et al. / High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy. Biomedical Imaging and Sensing Conference 2020. editor / Toyohiko Yatagai ; Yoshihisa Aizu ; Osamu Matoba ; Yasuhiro Awatsuji ; Yuan Luo. SPIE, 2020. (Proceedings of SPIE - The International Society for Optical Engineering).

@inproceedings{e1babac11b1241549771ad0db77cf39b,

title = "High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy",

abstract = "We used only a narrow range, 1397-1501 cm-1, for high-throughput analysis of cancerous and noncancerous human cell lines by spontaneous Raman microscopy. With baseline-corrected cellwise spectra in this range, two cell lines were discriminated at accuracy higher than 90%. This narrowband measurement allowed reduction of the signal readout time by 24-folds in comparison to a correspondent wideband measurement detecting 536-3132 cm-1, enabling cell analysis at 2.5 cells/min. To further improve the throughput, we employed detector binning, which allowed reduction not just of the readout time but also of the signal accumulation time with maintaining signal-to-background ratio and the accuracy. Improvement of the imaging speed by this approach reached at 4-folds, enabling a high-throughout analysis at 10 cells/min.",

keywords = "Cancer detection, Discriminant analysis, Narrowband, Raman microscopy, Spectral resolution",

author = "Yasuaki Kumamoto and Kentaro Mochizuki and Kosuke Hashimoto and Yoshinori Harada and Hideo Tanaka and Katsumasa Fujita",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; 6th Biomedical Imaging and Sensing Conference ; Conference date: 20-04-2020 Through 24-04-2020",

year = "2020",

doi = "10.1117/12.2573223",

language = "英語",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE",

editor = "Toyohiko Yatagai and Yoshihisa Aizu and Osamu Matoba and Yasuhiro Awatsuji and Yuan Luo",

booktitle = "Biomedical Imaging and Sensing Conference 2020",

}

Kumamoto, Y, Mochizuki, K, Hashimoto, K, Harada, Y, Tanaka, H & Fujita, K 2020, High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy. in T Yatagai, Y Aizu, O Matoba, Y Awatsuji & Y Luo (eds), Biomedical Imaging and Sensing Conference 2020., 1152112, Proceedings of SPIE - The International Society for Optical Engineering, vol. 11521, SPIE, 6th Biomedical Imaging and Sensing Conference, Yokohama, Japan, 2020/04/20. https://doi.org/10.1117/12.2573223

High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy. / Kumamoto, Yasuaki; Mochizuki, Kentaro; Hashimoto, Kosuke et al.
Biomedical Imaging and Sensing Conference 2020. ed. / Toyohiko Yatagai; Yoshihisa Aizu; Osamu Matoba; Yasuhiro Awatsuji; Yuan Luo. SPIE, 2020. 1152112 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11521).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy

AU - Kumamoto, Yasuaki

AU - Mochizuki, Kentaro

AU - Hashimoto, Kosuke

AU - Harada, Yoshinori

AU - Tanaka, Hideo

AU - Fujita, Katsumasa

N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2020

Y1 - 2020

N2 - We used only a narrow range, 1397-1501 cm-1, for high-throughput analysis of cancerous and noncancerous human cell lines by spontaneous Raman microscopy. With baseline-corrected cellwise spectra in this range, two cell lines were discriminated at accuracy higher than 90%. This narrowband measurement allowed reduction of the signal readout time by 24-folds in comparison to a correspondent wideband measurement detecting 536-3132 cm-1, enabling cell analysis at 2.5 cells/min. To further improve the throughput, we employed detector binning, which allowed reduction not just of the readout time but also of the signal accumulation time with maintaining signal-to-background ratio and the accuracy. Improvement of the imaging speed by this approach reached at 4-folds, enabling a high-throughout analysis at 10 cells/min.

AB - We used only a narrow range, 1397-1501 cm-1, for high-throughput analysis of cancerous and noncancerous human cell lines by spontaneous Raman microscopy. With baseline-corrected cellwise spectra in this range, two cell lines were discriminated at accuracy higher than 90%. This narrowband measurement allowed reduction of the signal readout time by 24-folds in comparison to a correspondent wideband measurement detecting 536-3132 cm-1, enabling cell analysis at 2.5 cells/min. To further improve the throughput, we employed detector binning, which allowed reduction not just of the readout time but also of the signal accumulation time with maintaining signal-to-background ratio and the accuracy. Improvement of the imaging speed by this approach reached at 4-folds, enabling a high-throughout analysis at 10 cells/min.

KW - Cancer detection

KW - Discriminant analysis

KW - Narrowband

KW - Raman microscopy

KW - Spectral resolution

UR - http://www.scopus.com/inward/record.url?scp=85087993203&partnerID=8YFLogxK

U2 - 10.1117/12.2573223

DO - 10.1117/12.2573223

M3 - 会議への寄与

AN - SCOPUS:85087993203

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Biomedical Imaging and Sensing Conference 2020

A2 - Yatagai, Toyohiko

A2 - Aizu, Yoshihisa

A2 - Matoba, Osamu

A2 - Awatsuji, Yasuhiro

A2 - Luo, Yuan

PB - SPIE

T2 - 6th Biomedical Imaging and Sensing Conference

Y2 - 20 April 2020 through 24 April 2020

ER -

Kumamoto Y, Mochizuki K, Hashimoto K, Harada Y, Tanaka H, Fujita K. High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy. In Yatagai T, Aizu Y, Matoba O, Awatsuji Y, Luo Y, editors, Biomedical Imaging and Sensing Conference 2020. SPIE. 2020. 1152112. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2573223

High-throughput discrimination of cancerous and noncancerous human cell lines by high-speed spontaneous Raman microscopy

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Fingerprint

Cite this