抄録
The development of new three-dimensional (3D) cell culture system that maintains the physiologically relevant signals of hepatocytes is essential in drug discovery and tissue engineering research. Conventional two-dimensional (2D) culture yields cell growth, proliferation, and differentiation. However, gene expression and signaling profiles can be different from in vivo environment. Here, we report the fabrication of a 3D culture system using an artificial scaffold and our custommade inkjet 3D bioprinter as a new strategy for studying liverspecific functions of hepatocytes. We built a 3D culture platform for hepatocytes-attachment and formation of cell monolayer by interacting the galactose chain of galactosylated alginate gel (GA-gel) with asialoglycoprotein receptor (ASGPR) of hepatocytes. The 3D geometrical arrangement of cells was controlled by using 3D bioprinter, and cell polarity was controlled with the galactosylated hydrogels. The fabricated GA-gel was able to successfully promote adhesion of hepatocytes. To observe liver-specific functions and to mimic hepatic cord, an additional parallel layer of hepatocytes was generated using two gel sheets. These results indicated that GA-gel biomimetic matrices can be used as a 3D culture systemthat could be effective for the engineering of liver tissues.
本文言語 | 英語 |
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ページ(範囲) | 1583-1592 |
ページ数 | 10 |
ジャーナル | Journal of Biomedical Materials Research - Part A |
巻 | 105 |
号 | 6 |
DOI | |
出版ステータス | 出版済み - 2017/06/01 |
ASJC Scopus 主題領域
- セラミックおよび複合材料
- 生体材料
- 生体医工学
- 金属および合金