Electrochemical genotyping by using two ferrocene/isoquinoline connected DNA probes with different redox potentials on a single electrode

Reona Ikeda, Satoshi Kitagawa, Junya Chiba, Masahiko Inouye*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

16 被引用数 (SciVal)

抄録

An electrochemical genotyping by using two ferrocene/isoquinoline-connected DNA probes with different redox potentials on a single electrode was investigated. A solution of 100μm Iq*-ADH7 phosphate buffer was placed on a commercially available gold electrode, which was cleaned. The electrode was kept in a closed container under high humidity for 90 mm at room temperature. After rinsed with a buffer solution, the gold electrode modified with DNA probes was soaked in a 1 mM 6-mercaptohexan 1-0l solution of a buffer solution for 90 mm at room temperature, and then thoroughly washed with Milli-Q water and the buffer solution successively. The mismatched duplex Iq*1-2 was found to reveal an enhanced bending elastically compared with the matched Iq*1-1 as the duplexes from Iq1 did. This electrochemical genotyping could be conducted accurately and rapidly by using the two DNA probes, which possess different redox potentials.

本文言語英語
ページ(範囲)7048-7051
ページ数4
ジャーナルChemistry - A European Journal
15
29
DOI
出版ステータス出版済み - 2009/07/20

ASJC Scopus 主題領域

  • 触媒
  • 有機化学

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