TY - JOUR
T1 - Developmental regulation of gene expression for the MPTPδ isoforms in the central nervous system and the immune system
AU - Mizuno, Kazuya
AU - Hasegawa, Kiminori
AU - Ogimoto, Mami
AU - Katagiri, Tatsuo
AU - Yakura, Hidetaka
N1 - Funding Information:
Acknowledgements: We are grateful to Dr. Tomoyuki Ichikawa and Ms. Kyoko Ajiki for technical advice on in situ hybridization. This work was supported in part by grants from the Ministry of Education, Science and Culture, the Immunodiagnostic Laboratory, and the Tokyo Biochemical Research Foundation.
PY - 1994/12/5
Y1 - 1994/12/5
N2 - MPTPδ is a murine transmembrane protein tyrosine phosphatase which has three isoforms, types A-C, differing in the structure of the extracellular regions. In this study, we examined MPTPδ isoform expression in the brain and the immune system at discrete developmental or differentiation stages. RT-PCR analysis demonstrated that another isoform, type D, is transcribed from the MPTPδ gene. In the brain, only type D was expressed until postnatal day 7 (P7), but after P14, all four isoforms were detected. In contrast, the spleen, thymus and all the hematopoietic cell lines examined express only types B and C isoforms. An in situ hybridization study showed that MPTPδ mRNA is diffusely expressed throughout the spleen, but its expression in the thymus is restricted to the medullary region.
AB - MPTPδ is a murine transmembrane protein tyrosine phosphatase which has three isoforms, types A-C, differing in the structure of the extracellular regions. In this study, we examined MPTPδ isoform expression in the brain and the immune system at discrete developmental or differentiation stages. RT-PCR analysis demonstrated that another isoform, type D, is transcribed from the MPTPδ gene. In the brain, only type D was expressed until postnatal day 7 (P7), but after P14, all four isoforms were detected. In contrast, the spleen, thymus and all the hematopoietic cell lines examined express only types B and C isoforms. An in situ hybridization study showed that MPTPδ mRNA is diffusely expressed throughout the spleen, but its expression in the thymus is restricted to the medullary region.
KW - Cell differentiation
KW - Isoform
KW - Protein tyrosine phosphatase
UR - http://www.scopus.com/inward/record.url?scp=0027943341&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(94)01188-5
DO - 10.1016/0014-5793(94)01188-5
M3 - 学術論文
C2 - 7988677
AN - SCOPUS:0027943341
SN - 0014-5793
VL - 355
SP - 223
EP - 228
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -