Developmental expression of the SRF co-activator MAL in brain: Role in regulating dendritic morphology

Jun Shiota, Mitsuru Ishikawa, Hiroyuki Sakagami, Masaaki Tsuda, Jay M. Baraban, Akiko Tabuchi*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

35 被引用数 (Scopus)

抄録

The dynamic changes in dendritic morphology displayed by developing and mature neurons have stimulated interest in deciphering the signaling pathways involved. Recent studies have identified megakaryocytic acute leukemia (MAL), a serum response factor (SRF) co-activator, as a key component of a signaling pathway linking changes in the actin cytoskeleton to SRF-mediated transcription. To help define the role of this pathway in regulating dendritic morphology, we have characterized the pattern of MAL expression in the developing and adult brain, and have examined its role in regulating dendritic morphology in cultured cortical neurons. In histological studies of mouse brain, we found prominent expression of MAL in neurons in adult hippocampus and cerebral cortex. MAL immunostaining revealed localization of this protein in neuronal cell bodies and apical dendrites. During development, an increase in MAL expression occurs during the second post-natal week. Expression of dominant negative MAL constructs or MAL siRNA in cortical neurons grown in primary culture reduces the number of dendritic processes and decreases the basal level of SRF-mediated transcription. Taken together, these findings indicate that the MAL-SRF signaling pathway plays a key role in regulating dendritic morphology.

本文言語英語
ページ(範囲)1778-1788
ページ数11
ジャーナルJournal of Neurochemistry
98
6
DOI
出版ステータス出版済み - 2006/09

ASJC Scopus 主題領域

  • 生化学
  • 細胞および分子神経科学

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