@article{0335f8d31c314d38bf44a39e32dde6b8,
title = "Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression",
abstract = "Two near full-length cDNA clones (LEC4H-1 and LEC4H-2) encoding plant cytochrome P450 were isolated by screening a cDNA library constructed from shikonin-producing Lithospermum erythrorhizon callus cultures. The deduced amino acid sequences of LEC4H-1 and LEC4H-2 exhibited 91% identity and were highly homologous to various cinnamic acid 4-hydroxylase (C4H) sequences from higher plants. Expression studies in yeast confirmed that the P450 clones encode C4H. The level of C4H transcripts in the cultured cells was un-affected by environmental factors, including light irradiation, supply of auxin and methyl jasmonate addition, indicating that transcription of these genes is constitutive and not involved in the regulation of shikonin and rosmarinic acid biosynthesis by these factors.",
keywords = "CDNA cloning, Cinnamic acid 4-hydroxylase, Lithospermum erythrorhizon, Rosmarinic acid, Shikonin",
author = "Y. Yamamura and Y. Ogihara and H. Mizukami",
note = "Funding Information: Acknowledgements We wish to thank Dr. K. Yazaki, Kyoto University, for providing us with the information on the sequences of LEPAL and LEDI-2 cDNAs and Dr. N. Goto, Sendai Arabidopsis Seed Stock Center, Miyagi University of Education, for providing us with the seeds of Arabidopsis thaliana (ecotype Columbia). We also thank Prof. B.E. Ellis, University of British Columbia, for reviewing the manuscript. The present work was supported in part by the Grant-in-Aid for Scientific Research and the Grant-in-Aid for High-Tech Research Center Project from the Ministry of Education, Science, Sports and Culture of Japan.",
year = "2001",
doi = "10.1007/s002990100373",
language = "英語",
volume = "20",
pages = "655--662",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "7",
}