Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression

Y. Yamamura; Y. Ogihara; H. Mizukami

doi:10.1007/s002990100373

Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression

Y. Yamamura, Y. Ogihara, H. Mizukami^*

^*Corresponding author for this work

Plant Resource Sciences

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Two near full-length cDNA clones (LEC4H-1 and LEC4H-2) encoding plant cytochrome P450 were isolated by screening a cDNA library constructed from shikonin-producing Lithospermum erythrorhizon callus cultures. The deduced amino acid sequences of LEC4H-1 and LEC4H-2 exhibited 91% identity and were highly homologous to various cinnamic acid 4-hydroxylase (C4H) sequences from higher plants. Expression studies in yeast confirmed that the P450 clones encode C4H. The level of C4H transcripts in the cultured cells was un-affected by environmental factors, including light irradiation, supply of auxin and methyl jasmonate addition, indicating that transcription of these genes is constitutive and not involved in the regulation of shikonin and rosmarinic acid biosynthesis by these factors.

Original language	English
Pages (from-to)	655-662
Number of pages	8
Journal	Plant Cell Reports
Volume	20
Issue number	7
DOIs	https://doi.org/10.1007/s002990100373
State	Published - 2001

Keywords

CDNA cloning
Cinnamic acid 4-hydroxylase
Lithospermum erythrorhizon
Rosmarinic acid
Shikonin

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s002990100373

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@article{0335f8d31c314d38bf44a39e32dde6b8,

title = "Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression",

abstract = "Two near full-length cDNA clones (LEC4H-1 and LEC4H-2) encoding plant cytochrome P450 were isolated by screening a cDNA library constructed from shikonin-producing Lithospermum erythrorhizon callus cultures. The deduced amino acid sequences of LEC4H-1 and LEC4H-2 exhibited 91% identity and were highly homologous to various cinnamic acid 4-hydroxylase (C4H) sequences from higher plants. Expression studies in yeast confirmed that the P450 clones encode C4H. The level of C4H transcripts in the cultured cells was un-affected by environmental factors, including light irradiation, supply of auxin and methyl jasmonate addition, indicating that transcription of these genes is constitutive and not involved in the regulation of shikonin and rosmarinic acid biosynthesis by these factors.",

keywords = "CDNA cloning, Cinnamic acid 4-hydroxylase, Lithospermum erythrorhizon, Rosmarinic acid, Shikonin",

author = "Y. Yamamura and Y. Ogihara and H. Mizukami",

note = "Funding Information: Acknowledgements We wish to thank Dr. K. Yazaki, Kyoto University, for providing us with the information on the sequences of LEPAL and LEDI-2 cDNAs and Dr. N. Goto, Sendai Arabidopsis Seed Stock Center, Miyagi University of Education, for providing us with the seeds of Arabidopsis thaliana (ecotype Columbia). We also thank Prof. B.E. Ellis, University of British Columbia, for reviewing the manuscript. The present work was supported in part by the Grant-in-Aid for Scientific Research and the Grant-in-Aid for High-Tech Research Center Project from the Ministry of Education, Science, Sports and Culture of Japan.",

year = "2001",

doi = "10.1007/s002990100373",

language = "英語",

volume = "20",

pages = "655--662",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "7",

}

TY - JOUR

T1 - Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon

T2 - cDNA cloning and gene expression

AU - Yamamura, Y.

AU - Ogihara, Y.

AU - Mizukami, H.

N1 - Funding Information: Acknowledgements We wish to thank Dr. K. Yazaki, Kyoto University, for providing us with the information on the sequences of LEPAL and LEDI-2 cDNAs and Dr. N. Goto, Sendai Arabidopsis Seed Stock Center, Miyagi University of Education, for providing us with the seeds of Arabidopsis thaliana (ecotype Columbia). We also thank Prof. B.E. Ellis, University of British Columbia, for reviewing the manuscript. The present work was supported in part by the Grant-in-Aid for Scientific Research and the Grant-in-Aid for High-Tech Research Center Project from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 2001

Y1 - 2001

N2 - Two near full-length cDNA clones (LEC4H-1 and LEC4H-2) encoding plant cytochrome P450 were isolated by screening a cDNA library constructed from shikonin-producing Lithospermum erythrorhizon callus cultures. The deduced amino acid sequences of LEC4H-1 and LEC4H-2 exhibited 91% identity and were highly homologous to various cinnamic acid 4-hydroxylase (C4H) sequences from higher plants. Expression studies in yeast confirmed that the P450 clones encode C4H. The level of C4H transcripts in the cultured cells was un-affected by environmental factors, including light irradiation, supply of auxin and methyl jasmonate addition, indicating that transcription of these genes is constitutive and not involved in the regulation of shikonin and rosmarinic acid biosynthesis by these factors.

AB - Two near full-length cDNA clones (LEC4H-1 and LEC4H-2) encoding plant cytochrome P450 were isolated by screening a cDNA library constructed from shikonin-producing Lithospermum erythrorhizon callus cultures. The deduced amino acid sequences of LEC4H-1 and LEC4H-2 exhibited 91% identity and were highly homologous to various cinnamic acid 4-hydroxylase (C4H) sequences from higher plants. Expression studies in yeast confirmed that the P450 clones encode C4H. The level of C4H transcripts in the cultured cells was un-affected by environmental factors, including light irradiation, supply of auxin and methyl jasmonate addition, indicating that transcription of these genes is constitutive and not involved in the regulation of shikonin and rosmarinic acid biosynthesis by these factors.

KW - CDNA cloning

KW - Cinnamic acid 4-hydroxylase

KW - Lithospermum erythrorhizon

KW - Rosmarinic acid

KW - Shikonin

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DO - 10.1007/s002990100373

M3 - 学術論文

AN - SCOPUS:0034777910

SN - 0721-7714

VL - 20

SP - 655

EP - 662

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 7

ER -

Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression

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Keywords

ASJC Scopus subject areas

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