Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein

Miyuki Kimura, Kazutaka Egawa, Tatsuhiko Ozawa, Hiroyuki Kishi, Masayuki Shimojima, Satoshi Taniguchi, Shuetsu Fukushi, Hikaru Fujii, Hiroshi Yamada, Long Tan, Kaori Sano, Harutaka Katano, Tadaki Suzuki, Shigeru Morikawa, Masayuki Saijo, Hideki Tani*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

11 被引用数 (Scopus)

抄録

The heartland virus (HRTV) is a novel phlebovirus that causes severe infections in the USA and closely related to the severe fever thrombocytopenia syndrome virus (SFTSV), a causative agent for SFTS in Asia. The entry mechanisms of HRTV remain unclear. Here, we developed the pseudotyped vesicular stomatitis virus bearing the HRTV glycoprotein (GP) (HRTVpv), and the antigenicity and the entry mechanisms of HRTV were analyzed. HRTVpv was neutralized by anti-SFTSV Gc antibody, but not the anti-SFTSV Gn antibodies. Entry of HRTVpv to cells was inhibited by bafilomycin A1 and dynasore, and but it was enhanced in cells overexpressed with C-type lectins. Production of infectious HRTVpv and SFTSVpv was reduced by Nn-DNJ, α-glucosidase inhibitor. The entry of HRTV occurs via pH- and dynamin-dependent endocytosis. Furthermore, Nn-DNJ may be a possible therapeutic agent against HRTV and SFTSV.

本文言語英語
ページ(範囲)124-132
ページ数9
ジャーナルVirology
556
DOI
出版ステータス出版済み - 2021/04

ASJC Scopus 主題領域

  • ウイルス学

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