Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein

Miyuki Kimura; Kazutaka Egawa; Tatsuhiko Ozawa; Hiroyuki Kishi; Masayuki Shimojima; Satoshi Taniguchi; Shuetsu Fukushi; Hikaru Fujii; Hiroshi Yamada; Long Tan; Kaori Sano; Harutaka Katano; Tadaki Suzuki; Shigeru Morikawa; Masayuki Saijo; Hideki Tani

doi:10.1016/j.virol.2020.10.006

Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein

Miyuki Kimura, Kazutaka Egawa, Tatsuhiko Ozawa, Hiroyuki Kishi, Masayuki Shimojima, Satoshi Taniguchi, Shuetsu Fukushi, Hikaru Fujii, Hiroshi Yamada, Long Tan, Kaori Sano, Harutaka Katano, Tadaki Suzuki, Shigeru Morikawa, Masayuki Saijo, Hideki Tani^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The heartland virus (HRTV) is a novel phlebovirus that causes severe infections in the USA and closely related to the severe fever thrombocytopenia syndrome virus (SFTSV), a causative agent for SFTS in Asia. The entry mechanisms of HRTV remain unclear. Here, we developed the pseudotyped vesicular stomatitis virus bearing the HRTV glycoprotein (GP) (HRTVpv), and the antigenicity and the entry mechanisms of HRTV were analyzed. HRTVpv was neutralized by anti-SFTSV Gc antibody, but not the anti-SFTSV Gn antibodies. Entry of HRTVpv to cells was inhibited by bafilomycin A1 and dynasore, and but it was enhanced in cells overexpressed with C-type lectins. Production of infectious HRTVpv and SFTSVpv was reduced by Nn-DNJ, α-glucosidase inhibitor. The entry of HRTV occurs via pH- and dynamin-dependent endocytosis. Furthermore, Nn-DNJ may be a possible therapeutic agent against HRTV and SFTSV.

Original language	English
Pages (from-to)	124-132
Number of pages	9
Journal	Virology
Volume	556
DOIs	https://doi.org/10.1016/j.virol.2020.10.006
State	Published - 2021/04

Keywords

Antigenicity
Glycoprotein
Heartland virus (HRTV)
Host cell entry
Pseudotyped virus
Severe fever thrombocytopenia syndrome virus (SFTSV)

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2020.10.006

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Kimura, M., Egawa, K., Ozawa, T., Kishi, H., Shimojima, M., Taniguchi, S., Fukushi, S., Fujii, H., Yamada, H., Tan, L., Sano, K., Katano, H., Suzuki, T., Morikawa, S., Saijo, M., & Tani, H. (2021). Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein. Virology, 556, 124-132. https://doi.org/10.1016/j.virol.2020.10.006

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title = "Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein",

abstract = "The heartland virus (HRTV) is a novel phlebovirus that causes severe infections in the USA and closely related to the severe fever thrombocytopenia syndrome virus (SFTSV), a causative agent for SFTS in Asia. The entry mechanisms of HRTV remain unclear. Here, we developed the pseudotyped vesicular stomatitis virus bearing the HRTV glycoprotein (GP) (HRTVpv), and the antigenicity and the entry mechanisms of HRTV were analyzed. HRTVpv was neutralized by anti-SFTSV Gc antibody, but not the anti-SFTSV Gn antibodies. Entry of HRTVpv to cells was inhibited by bafilomycin A1 and dynasore, and but it was enhanced in cells overexpressed with C-type lectins. Production of infectious HRTVpv and SFTSVpv was reduced by Nn-DNJ, α-glucosidase inhibitor. The entry of HRTV occurs via pH- and dynamin-dependent endocytosis. Furthermore, Nn-DNJ may be a possible therapeutic agent against HRTV and SFTSV.",

keywords = "Antigenicity, Glycoprotein, Heartland virus (HRTV), Host cell entry, Pseudotyped virus, Severe fever thrombocytopenia syndrome virus (SFTSV)",

author = "Miyuki Kimura and Kazutaka Egawa and Tatsuhiko Ozawa and Hiroyuki Kishi and Masayuki Shimojima and Satoshi Taniguchi and Shuetsu Fukushi and Hikaru Fujii and Hiroshi Yamada and Long Tan and Kaori Sano and Harutaka Katano and Tadaki Suzuki and Shigeru Morikawa and Masayuki Saijo and Hideki Tani",

note = "Publisher Copyright: {\textcopyright} 2020",

year = "2021",

month = apr,

doi = "10.1016/j.virol.2020.10.006",

language = "英語",

volume = "556",

pages = "124--132",

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T1 - Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein

AU - Kimura, Miyuki

AU - Egawa, Kazutaka

AU - Ozawa, Tatsuhiko

AU - Kishi, Hiroyuki

AU - Shimojima, Masayuki

AU - Taniguchi, Satoshi

AU - Fukushi, Shuetsu

AU - Fujii, Hikaru

AU - Yamada, Hiroshi

AU - Tan, Long

AU - Sano, Kaori

AU - Katano, Harutaka

AU - Suzuki, Tadaki

AU - Morikawa, Shigeru

AU - Saijo, Masayuki

AU - Tani, Hideki

PY - 2021/4

Y1 - 2021/4

N2 - The heartland virus (HRTV) is a novel phlebovirus that causes severe infections in the USA and closely related to the severe fever thrombocytopenia syndrome virus (SFTSV), a causative agent for SFTS in Asia. The entry mechanisms of HRTV remain unclear. Here, we developed the pseudotyped vesicular stomatitis virus bearing the HRTV glycoprotein (GP) (HRTVpv), and the antigenicity and the entry mechanisms of HRTV were analyzed. HRTVpv was neutralized by anti-SFTSV Gc antibody, but not the anti-SFTSV Gn antibodies. Entry of HRTVpv to cells was inhibited by bafilomycin A1 and dynasore, and but it was enhanced in cells overexpressed with C-type lectins. Production of infectious HRTVpv and SFTSVpv was reduced by Nn-DNJ, α-glucosidase inhibitor. The entry of HRTV occurs via pH- and dynamin-dependent endocytosis. Furthermore, Nn-DNJ may be a possible therapeutic agent against HRTV and SFTSV.

AB - The heartland virus (HRTV) is a novel phlebovirus that causes severe infections in the USA and closely related to the severe fever thrombocytopenia syndrome virus (SFTSV), a causative agent for SFTS in Asia. The entry mechanisms of HRTV remain unclear. Here, we developed the pseudotyped vesicular stomatitis virus bearing the HRTV glycoprotein (GP) (HRTVpv), and the antigenicity and the entry mechanisms of HRTV were analyzed. HRTVpv was neutralized by anti-SFTSV Gc antibody, but not the anti-SFTSV Gn antibodies. Entry of HRTVpv to cells was inhibited by bafilomycin A1 and dynasore, and but it was enhanced in cells overexpressed with C-type lectins. Production of infectious HRTVpv and SFTSVpv was reduced by Nn-DNJ, α-glucosidase inhibitor. The entry of HRTV occurs via pH- and dynamin-dependent endocytosis. Furthermore, Nn-DNJ may be a possible therapeutic agent against HRTV and SFTSV.

KW - Antigenicity

KW - Glycoprotein

KW - Heartland virus (HRTV)

KW - Host cell entry

KW - Pseudotyped virus

KW - Severe fever thrombocytopenia syndrome virus (SFTSV)

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U2 - 10.1016/j.virol.2020.10.006

DO - 10.1016/j.virol.2020.10.006

M3 - 学術論文

C2 - 33561699

AN - SCOPUS:85100436482

SN - 0042-6822

VL - 556

SP - 124

EP - 132

JO - Virology

JF - Virology

ER -

Characterization of pseudotyped vesicular stomatitis virus bearing the heartland virus envelope glycoprotein

Abstract

Keywords

ASJC Scopus subject areas

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