TY - JOUR
T1 - Characterization of laminin isoforms in human amnion
AU - Takashima, Seiji
AU - Yasuo, Masanori
AU - Sanzen, Noriko
AU - Sekiguchi, Kiyotoshi
AU - Okabe, Motonori
AU - Yoshida, Toshiko
AU - Toda, Ayaka
AU - Nikaido, Toshio
PY - 2008/4
Y1 - 2008/4
N2 - Epithelial cells of the human amnion have been reported to possess similar functions to many types of cells, such as hepatocytes, neurons, and pancreatic β-cells. We reported previously that one of the hepatocyte-like functions of human amniotic epithelial cells was reinforced by the presence of basement membrane components. Laminin is one of the main components of the basement membrane; it critically contributes to cell differentiation. Laminin has several heterotrimer isoforms composed of an α-, a β-, and a γ-chain, and each type of chain has several types of subunit chains: α1-5, β1-3, and γ1-3. In this study, we characterized the laminin subunit chains in human amnion. Laminin is produced and secreted from adjacent epithelial cells, and therefore, the gene expression of laminin subunit chains in human amniotic epithelial cells was investigated by RT-PCR. Their localization was examined by immunohistochemical staining of frozen sections. The findings suggested that the basement membrane of the human amnion contains a broad spectrum of laminin isoforms, laminin-2, -4, -5, -6, -7, -10, -11. These findings will provide clues not only for understanding the physiological roles of the amnion and hAECs, but also for applying this tissue as a source of donor cells for cell transplantation therapy.
AB - Epithelial cells of the human amnion have been reported to possess similar functions to many types of cells, such as hepatocytes, neurons, and pancreatic β-cells. We reported previously that one of the hepatocyte-like functions of human amniotic epithelial cells was reinforced by the presence of basement membrane components. Laminin is one of the main components of the basement membrane; it critically contributes to cell differentiation. Laminin has several heterotrimer isoforms composed of an α-, a β-, and a γ-chain, and each type of chain has several types of subunit chains: α1-5, β1-3, and γ1-3. In this study, we characterized the laminin subunit chains in human amnion. Laminin is produced and secreted from adjacent epithelial cells, and therefore, the gene expression of laminin subunit chains in human amniotic epithelial cells was investigated by RT-PCR. Their localization was examined by immunohistochemical staining of frozen sections. The findings suggested that the basement membrane of the human amnion contains a broad spectrum of laminin isoforms, laminin-2, -4, -5, -6, -7, -10, -11. These findings will provide clues not only for understanding the physiological roles of the amnion and hAECs, but also for applying this tissue as a source of donor cells for cell transplantation therapy.
KW - Amnion
KW - Basement membrane
KW - Laminin
KW - Stem cell
UR - http://www.scopus.com/inward/record.url?scp=39749120398&partnerID=8YFLogxK
U2 - 10.1016/j.tice.2007.09.001
DO - 10.1016/j.tice.2007.09.001
M3 - 学術論文
C2 - 18291433
AN - SCOPUS:39749120398
SN - 0040-8166
VL - 40
SP - 75
EP - 81
JO - Tissue and Cell
JF - Tissue and Cell
IS - 2
ER -