Characterization of amniotic stem cells

Chika Koike, Kaixuan Zhou, Yuji Takeda, Moustafa Fathy, Motonori Okabe, Toshiko Yoshida, Yukio Nakamura, Yukio Kato, Toshio Nikaido*

*この論文の責任著者

研究成果: ジャーナルへの寄稿学術論文査読

68 被引用数 (Scopus)

抄録

The amnion membrane is developed from embryo-derived cells, and amniotic cells have been shown to exhibit multidifferentiation potential. These cells represent a desirable source for stem cells for a variety of reasons. However, to date very few molecular analyses of amnion-derived cells have been reported, and efficient markers for isolating the stem cells remain unclear. This paper assesses the characterization of amnion-derived cells as stem cells by examining stemness marker expressions for amnion-derived epithelial cells and mesenchymal cells by flow cytometry, immunocytochemistry, and quantitative PCR. Flow cytometry revealed that amnion epithelial cells expressed CD133, CD 271, and TRA-1-60, whereas mecenchymal cells expressed CD44, CD73, CD90, and CD105. Immunohistochemistry showed that both cells expressed the stemness markers Oct3/4, Sox2, Klf4, and SSEA4. Stemness genes' expression in amnion epithelial cells, mesenchymal cells, fibroblast, bone marrow-derived mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSCs) was compared by quantitative reverse-transcription polymerase chain reaction (RT-PCR). Amnion-derived epithelial cells and mesenchymal cells expressed Oct3/4, Nanog, and Klf4 more than bone marrow-derived MSCs. The sorted TRA1-60-positive cells expressed Oct3/4, Nanog, and Klf4 more than unsorted cells or TRA1-60-negative cells. TRA1-60 can be a marker for isolating amnion epithelial stem cells.

本文言語英語
ページ(範囲)298-305
ページ数8
ジャーナルCellular Reprogramming
16
4
DOI
出版ステータス出版済み - 2014/08/01

ASJC Scopus 主題領域

  • バイオテクノロジー
  • 発生生物学
  • 細胞生物学

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